Halmepuro L, Salvaggio J E, Lehrer S B
Department of Medicine, Tulane Medical Center, New Orleans, La.
Int Arch Allergy Appl Immunol. 1987;84(2):165-72. doi: 10.1159/000234418.
Antigenic and allergenic components in crawfish and lobster extracts were studied using crossed immunoelectrophoretic techniques. Crossed immunoelectrophoresis with rabbit antisera revealed 23 antigens in crawfish and 17 antigens in lobster extracts. Both extracts exhibited structural similarities in antigens mutually and with other crustacea in cross-line immunoelectrophoresis. Crossed radioimmunoelectrophoresis (CRIE) demonstrated 6 crawfish and 4 lobster allergens when individual or pooled sera from radioallergosorbent test (RAST)-positive crustacea-sensitive subjects were used. Since radiostaining was also observed with sera from RAST-negative nonsensitive subjects, specificity of IgE binding was tested using CRIE-inhibition. Preincubation of RAST-positive sera with crawfish or lobster extract decreased radiostaining in CRIE, while no changes occurred when using control sera. These results confirmed the presence of IgE-mediated mechanisms in seafood allergy and demonstrated a number of shared antigenic determinants among crustacea allergens.
采用交叉免疫电泳技术研究了小龙虾和龙虾提取物中的抗原和变应原成分。用兔抗血清进行交叉免疫电泳显示，小龙虾提取物中有23种抗原，龙虾提取物中有17种抗原。在交叉线免疫电泳中，两种提取物在相互之间以及与其他甲壳类动物的抗原方面表现出结构相似性。当使用来自放射性变应原吸附试验（RAST）阳性的甲壳类动物敏感受试者的个体或混合血清时，交叉放射免疫电泳（CRIE）显示出6种小龙虾变应原和4种龙虾变应原。由于在RAST阴性的非敏感受试者的血清中也观察到放射染色，因此使用CRIE抑制试验来测试IgE结合的特异性。将RAST阳性血清与小龙虾或龙虾提取物预孵育可降低CRIE中的放射染色，而使用对照血清时则无变化。这些结果证实了海鲜过敏中IgE介导机制的存在，并证明了甲壳类动物变应原中有许多共同的抗原决定簇。
