Gujar Ramkailash, Gregori Giulia, Dolz-Marco Rosa, Muzi Alessio, Chhablani Jay, Fruttini Daniela, Mangoni Lorenzo, Rizzo Clara, Mariotti Cesare, Lupidi Marco
Cornea and Stem Cells Department, Dr. Shroff's Charity Eye Hospital, Daryaganj, New Delhi, India.
Ophthalmic Unit, Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Verona, Italy.
Int J Retina Vitreous. 2025 Mar 13;11(1):28. doi: 10.1186/s40942-025-00653-w.
This study aimed to determine the optimal OCT angiography (OCT-A) scanning pattern using the SPECTRALIS HRA-OCT2 device to evaluate macular microvasculature perfusion in healthy subjects.
Healthy subjects were imaged using the SPECTRALIS OCT-A Module (Heidelberg Engineering) with the following scanning protocols: 10ºX10º-512 ART 7 [P1], 10ºX10º-256 ART 5 [P2], 10ºX10º-512 ART 5 [P3], and 15ºX10º-256 ART 5 [P4], all centered on the macula. Vessel perfusion density (VPD) and vessel length density (VLD) of the superficial vascular complex (SVC) were calculated using ImageJ software to evaluate the differences between scanning patterns. Three additional 10ºx1º, ART 7 high-density images were also obtained using the in-built software (SP-X1701 Update 3, based on Heyex Software Version 1.9.215.0 H) in the macular area and the VPD and VLD for all the three10ºx1º pattern size images with the corresponding area of pattern 1 image[P1]. Two retinal specialists conducted a blind qualitative assessment of the foveal avascular zone and image quality.
Twenty eyes from 20 consecutive healthy patients were included in the study. The mean VPD for P1, P2, P3, and P4 were 35.60, 31.67, 31.18, and 31.16, respectively. Mean VLD for P1, P2, P3, and P4 were 7.54, 5.86, 6.74, and 4.40, respectively. Significant differences were found between P1 and the other patterns for both the VPD and VLD, but not between P2, P3, and P4. VPD and VLD for 10ºx1º high-density images were 33.20 and 4.61, respectively, with significant VLD differences compared to P1, but not for VPD. P1 scored the highest and P4 the lowest in the qualitative assessments.
The 10ºX10º-512 ART 7 pattern showed statistically significant qualitative superiority and appeared optimal for blood flow detection with reduced noise in quantitative assessments.
本研究旨在确定使用SPECTRALIS HRA - OCT2设备评估健康受试者黄斑部微血管灌注的最佳光学相干断层扫描血管造影（OCT - A）扫描模式。
使用SPECTRALIS OCT - A模块（海德堡工程公司）对健康受试者进行成像，采用以下扫描方案：10ºX10º - 512 ART 7 [P1]、10ºX10º - 256 ART 5 [P2]、10ºX10º - 512 ART 5 [P3]和15ºX10º - 256 ART 5 [P4]，均以黄斑为中心。使用ImageJ软件计算浅表血管复合体（SVC）的血管灌注密度（VPD）和血管长度密度（VLD），以评估扫描模式之间的差异。还使用黄斑区域内置软件（基于Heyex软件版本1.9.215.0 H的SP - X1701 Update 3）获取另外三张10ºx1º、ART 7的高密度图像，并计算这三张10ºx1º模式尺寸图像与模式1图像[P1]相应区域的VPD和VLD。两位视网膜专家对黄斑无血管区和图像质量进行了盲法定性评估。
本研究纳入了连续20例健康患者的20只眼。P1、P2、P3和P4的平均VPD分别为35.60、31.67、31.18和31.16。P1、P2、P3和P4的平均VLD分别为7.54、5.86、6.74和4.40。在VPD和VLD方面，P1与其他模式之间存在显著差异，但P2、P3和P4之间无显著差异。10ºx1º高密度图像的VPD和VLD分别为33.20和4.61，与P1相比，VLD存在显著差异，但VPD无显著差异。在定性评估中，P1得分最高，P4得分最低。
10ºX10º - 512 ART 7模式在统计学上显示出显著的定性优势，并且在定量评估中似乎是检测血流且噪声降低的最佳模式。
