Zhao Yancheng, Chang Deyuan, Zheng Yanxuan, Zhang Yuwei, Wang Yongjie, Bao Xiaokai, Sun Guohua, Feng Yanwei, Li Zan, Liu Xiumei, Yang Jianmin
School of Fisheries, Ludong University, Yantai, 264025, China.
Rushan Marine and Fishery Monitoring and Hazard Mitigation Center, Rushan, 264500, China.
BMC Genomics. 2025 Mar 17;26(1):262. doi: 10.1186/s12864-025-11418-y.
Sepia esculenta is one of the most abundant extant squid populations in Southeast Asia and is of interest due to its rapid reproductive rate and high commercial value. In recent years, with the rapid development of industrialization, issues such as global warming and heavy metal pollution in the oceans have emerged, posing a serious threat to the life activities of marine organisms. In this study, we used transcriptomic techniques to investigate the differences in Cu exposure immune responses in S. esculenta larvae under different temperature conditions. The enrichment of solute carrier family (SLC) genes and genes related to DNA replication and damage was significantly higher in the CuT group than in the Cu group. Functional enrichment analysis revealed that the FcγR-mediated phagocytosis and autophagy pathways were enriched in the CuT group. Based on the analysis of differentially expressed genes (DEGs) and functional enrichment results, we can preliminarily infer that the CuT group caused more severe disruption of intercellular ion transport and DNA replication and repair in larvae compared to the Cu group. This may have further interfered with the normal physiological activities of S. esculenta larvae. Overall, at high temperatures, Cu exposure induces a more intense inflammatory response. The results of this study provide a theoretical foundation for researchers to further understand the effects of environmental factors on the immunity of S. esculenta larvae, as well as preliminary insights into the enhanced toxic effects of metallic copper on aquatic organisms under high-temperature conditions.
曼氏无针乌贼是东南亚现存数量最多的鱿鱼种群之一，因其繁殖速度快和商业价值高而备受关注。近年来，随着工业化的快速发展，海洋中全球变暖和重金属污染等问题日益凸显，对海洋生物的生命活动构成了严重威胁。在本研究中，我们运用转录组技术，探究了不同温度条件下曼氏无针乌贼幼体对铜暴露的免疫反应差异。与铜组相比，铜温组中溶质载体家族（SLC）基因以及与DNA复制和损伤相关的基因富集程度显著更高。功能富集分析表明，铜温组中FcγR介导的吞噬作用和自噬途径得到了富集。基于差异表达基因（DEG）分析和功能富集结果，我们可以初步推断，与铜组相比，铜温组对幼体的细胞间离子转运以及DNA复制和修复造成了更严重的破坏。这可能进一步干扰了曼氏无针乌贼幼体的正常生理活动。总体而言，在高温条件下，铜暴露会引发更强烈的炎症反应。本研究结果为研究人员进一步了解环境因素对曼氏无针乌贼幼体免疫力的影响提供了理论基础，同时也为深入探究高温条件下金属铜对水生生物的增强毒性效应提供了初步见解。
