Liao Ying-Shu, Hong Yu-Ping, Chen Bo-Han, Wang You-Wun, Teng Ru-Hsiou, Chien Yung-Chen, Liang Shiu-Yun, Wei Hsiao Lun, Chang Jui-Hsien, Yang Ming-Hao, Tsao Chi-Sen, Chiou Chien-Shun
Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taichung, Taiwan.
Taipei City Hospital Renai Branch, Taipei City, Taiwan.
Microbiol Spectr. 2025 Mar 19;13(5):e0297924. doi: 10.1128/spectrum.02979-24.
We investigated emerging multidrug-resistant (MDR) serovar Agona in Taiwan. These MDR strains commonly harbored the efflux pump activator gene and up to 15 resistance genes, including , , , , , , , , , , ), , , , and ), within IncHI2-IncHI2A plasmids or diverse chromosomal resistance genomic islands (RGIs). These newly emerging MDR strains comprised 44.9% and 74.4% of . Agona isolates collected in 2023 and 2024, respectively. Through analysis of chromosomal insertion sites, we identified 11 distinct RGIs. Strains containing RGI_SA11 became predominant, comprising 59.2% of . Agona isolates collected in 2024.IMPORTANCEThe emergence and clonal expansion of MDR . Agona represent a growing public health concern. This study identifies a significant shift in resistance mechanisms, driven by chromosomally integrated resistance genomic islands (RGIs), which likely originated from IncHI2-IncHI2A plasmids. The chromosomal integration of antimicrobial resistance genes enhances the evolutionary fitness of these strains, facilitating their persistence and dissemination in both human and animal populations. These findings underscore the urgent need for enhanced surveillance, targeted interventions, and global antimicrobial stewardship to curb the spread of MDR pathogens and safeguard public health.
我们对台湾地区新出现的多重耐药（MDR）阿哥纳血清型进行了调查。这些MDR菌株通常携带外排泵激活基因以及多达15个耐药基因，包括（此处列举众多基因，原文未给出具体中文对应，无法准确翻译），存在于IncHI2-IncHI2A质粒或不同的染色体耐药基因组岛（RGIs）中。这些新出现的MDR菌株分别占2023年和2024年收集的阿哥纳血清型分离株的44.9%和74.4%。通过分析染色体插入位点，我们鉴定出11个不同的RGIs。含有RGI_SA11的菌株成为优势菌株，占2024年收集的阿哥纳血清型分离株的59.2%。
重要性
MDR阿哥纳血清型的出现和克隆扩增对公共卫生构成了日益严重的威胁。本研究确定了由染色体整合的耐药基因组岛（RGIs）驱动的耐药机制的重大转变，这些基因组岛可能起源于IncHI2-IncHI2A质粒。抗菌耐药基因的染色体整合增强了这些菌株的进化适应性，促进了它们在人类和动物群体中的持续存在和传播。这些发现强调了加强监测、针对性干预和全球抗菌药物管理以遏制MDR病原体传播和保障公众健康的迫切需求。
