Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China.
Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Biomater Sci. 2023 May 30;11(11):3813-3827. doi: 10.1039/d3bm00159h.
Cancer is a severe threat to human life and health and represents the main cause of death globally. Drug therapy is one of the primary means of treating cancer; however, most anticancer medications do not proceed beyond preclinical testing because the conditions of actual human tumors are not effectively mimicked by traditional tumor models. Hence, bionic tumor models must be developed to screen for anticancer drugs. Three-dimensional (3D) bioprinting technology can produce structures with built-in spatial and chemical complexity and models with accurately controlled structures, a homogeneous size and morphology, less variation across batches, and a more realistic tumor microenvironment (TME). This technology can also rapidly produce such models for high-throughput anticancer medication testing. This review describes 3D bioprinting methods, the use of bioinks in tumor models, and tumor model design strategies for building complex tumor microenvironment features using biological 3D printing technology. Moreover, the application of 3D bioprinting tumor models in drug screening is also discussed.
癌症是严重威胁人类生命和健康的疾病，也是全球主要的致死病因。药物治疗是癌症治疗的主要手段之一；然而，由于传统肿瘤模型无法有效模拟实际人类肿瘤的情况，大多数抗癌药物都无法通过临床前测试。因此，必须开发仿生肿瘤模型来筛选抗癌药物。三维（3D）生物打印技术可以制造出具有内置空间和化学复杂性的结构，以及具有精确控制结构、均匀尺寸和形态、批次间变化较小、更接近真实肿瘤微环境（TME）的模型。该技术还可以快速生成此类模型，以进行高通量抗癌药物测试。本文综述了 3D 生物打印方法、生物墨水在肿瘤模型中的应用，以及利用生物 3D 打印技术构建复杂肿瘤微环境特征的肿瘤模型设计策略。此外，还讨论了 3D 生物打印肿瘤模型在药物筛选中的应用。
