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大鼠和犬模型中用于精准细胞移植的机器人辅助立体定向显微注射方法

Robot-Assisted Stereotactic Microinjection Method for Precision Cell Transplantation in Rat and Canine Models.

作者信息

Han Deqiang, Chen Sichang, Wang Yuan, Wang Xueyao, Wang Xingzhe, Zheng Tianqi, Chen Zhiguo

机构信息

Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.

Department of Neurosurgery, Xuanwu Hospital Capital Medical University, Beijing, China.

出版信息

Cell Transplant. 2025 Jan-Dec;34:9636897251323351. doi: 10.1177/09636897251323351. Epub 2025 Mar 18.

DOI:10.1177/09636897251323351
PMID:40103182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11924096/
Abstract

Cell transplantation is a promising approach for addressing neurodegenerative conditions. In this study, we developed a robot-assisted stereotactic microinjection system for transplanting cells. We evaluated the factors that affect cellular graft viability and other properties, including the gauge of the syringe needle and the injection rate. We systematically compared the synchronous withdrawal injection (SWI) and fixed-point injection (FPI) procedures in agarose and rat brain models. assessments revealed superior dye dispersion with SWI compared to FPI, and analyses confirmed that SWI reduced the tissue injury and improved cell distribution in the striatum. We applied this robot-assisted technique to evaluate the accuracy and safety of cell transplantation in canine models. Overall, this strategy enhances the accuracy and safety of graft delivery, potentially improving outcomes and advancing therapeutic strategies for the clinical treatment of neurodegenerative disorders.

摘要

细胞移植是解决神经退行性疾病的一种有前景的方法。在本研究中，我们开发了一种用于细胞移植的机器人辅助立体定向显微注射系统。我们评估了影响细胞移植物活力和其他特性的因素，包括注射器针头的规格和注射速率。我们在琼脂糖和大鼠脑模型中系统地比较了同步回撤注射（SWI）和定点注射（FPI）程序。评估显示，与FPI相比，SWI的染料扩散效果更佳，分析证实SWI减少了组织损伤并改善了纹状体中的细胞分布。我们应用这种机器人辅助技术评估犬模型中细胞移植的准确性和安全性。总体而言，该策略提高了移植物递送的准确性和安全性，有可能改善治疗效果并推进神经退行性疾病临床治疗的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/5aad94897647/10.1177_09636897251323351-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/7a32ce25eb62/10.1177_09636897251323351-img2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/aa3d43c18452/10.1177_09636897251323351-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/41411ecbf805/10.1177_09636897251323351-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/f9c45b334048/10.1177_09636897251323351-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/d7a031783554/10.1177_09636897251323351-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/ff5dd5058e39/10.1177_09636897251323351-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/5aad94897647/10.1177_09636897251323351-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/7a32ce25eb62/10.1177_09636897251323351-img2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/aa3d43c18452/10.1177_09636897251323351-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/41411ecbf805/10.1177_09636897251323351-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/f9c45b334048/10.1177_09636897251323351-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/d7a031783554/10.1177_09636897251323351-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/ff5dd5058e39/10.1177_09636897251323351-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cba2/11924096/5aad94897647/10.1177_09636897251323351-fig6.jpg

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本文引用的文献

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Modulation of human induced neural stem cell-derived dopaminergic neurons by DREADD reveals therapeutic effects on a mouse model of Parkinson's disease.通过 DREADD 对人诱导多能干细胞源性多巴胺能神经元的调制揭示了其对帕金森病小鼠模型的治疗效果。
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Brain repair mechanisms after cell therapy for stroke.脑卒中介入治疗后的脑修复机制。
Brain. 2024 Oct 3;147(10):3286-3305. doi: 10.1093/brain/awae204.
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First-in-human transplantation of autologous induced neural stem cell-derived dopaminergic precursors to treat Parkinson's disease.
首次将自体诱导神经干细胞来源的多巴胺能前体细胞移植用于治疗帕金森病。
Sci Bull (Beijing). 2023 Nov 30;68(22):2700-2703. doi: 10.1016/j.scib.2023.10.020. Epub 2023 Oct 27.
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The 2020 Yearbook of Neurorestoratology.《2020年神经修复学年鉴》
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Human iPSC-derived midbrain organoids functionally integrate into striatum circuits and restore motor function in a mouse model of Parkinson's disease.人诱导多能干细胞衍生的中脑细胞球体在功能上整合到纹状体回路中，并在帕金森病小鼠模型中恢复运动功能。
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