Desai Shivaprasad Shivappa, Deepika Roy K A, Abishad Padikkamannil, Krishnan Rahul, Vinod Valil Kunjukunju, Karthikeyan Asha, Aswathi Plantharayil Bharathan, Juliet Sanis, Barbuddhe Sukhadeo Baliram, Rawool Deepak Bhiwa, Vergis Jess
Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University Wayanad 673 576 India
Department of Aquatic Animal Health Management, Faculty of Fisheries Science, Kerala University of Fisheries and Ocean Studies Panangad Kochi 682 506 India.
RSC Adv. 2025 Mar 17;15(11):8180-8188. doi: 10.1039/d5ra00509d.
This study aimed to synthesize and characterize silver-based metal-organic frameworks (Ag-MOFs) using 1,4-diazabicyclo[2.2.2]octane (DABCO) as the organic ligand and to assess their antibacterial and cytotoxic properties. The formation of Ag-MOF-D was confirmed by the appearance of a brown solution and a surface plasmon resonance peak at 394 nm in UV-vis spectroscopy. Fourier-transform infrared spectra showed characteristic peaks at 673, 705, 883, 1060, 1382, 1654, and 3250 cm. Powder X-ray diffraction patterns indicated a crystalline structure with peaks at 33°, 38°, 55°, and 66°, with an average particle size of 15.68 nm. Ag-MOF-D displayed thermal stability up to 650 °C with a residual mass of 91.50%. Scanning electron microscopy revealed spherical morphology with minimal aggregation, while energy-dispersive X-ray spectroscopy showed 88.64 wt% Ag. Transmission electron microscopy indicated mono-dispersed spherical particles with an average diameter of 10.47 ± 1.80 nm and a lattice fringe spacing of 0.19 nm. The type II isotherm and Brunauer-Emmett-Teller analysis suggested a mesoporous structure of Ag-MOF-D with a surface area of 5.3005 m g and an average pore diameter of 9.46 nm. Minimum inhibitory and minimum bactericidal concentration values against multi-drug-resistant bacterial strains ranged from 3.90 to 7.80 μM and 7.8 to 62.5 μM, respectively. cytotoxicity testing on Vero cell lines indicated a dose-dependent decrease in cell viability, with an IC value of 1.701 × 10 mg mL. These findings suggest that Ag-MOF-D holds potential for antibacterial applications and biocompatibility, with future opportunities for environmental and food safety applications.
本研究旨在以1,4-二氮杂双环[2.2.2]辛烷（DABCO）作为有机配体合成并表征银基金属有机框架（Ag-MOFs），并评估其抗菌和细胞毒性特性。紫外可见光谱中棕色溶液的出现以及394nm处的表面等离子体共振峰证实了Ag-MOF-D的形成。傅里叶变换红外光谱在673、705、883、1060、1382、1654和3250cm处显示出特征峰。粉末X射线衍射图谱表明其具有晶体结构，在33°、38°、55°和66°处有峰，平均粒径为15.68nm。Ag-MOF-D在高达650°C时显示出热稳定性，残余质量为91.50%。扫描电子显微镜显示出球形形态且聚集极少，而能量色散X射线光谱显示银含量为88.64 wt%。透射电子显微镜表明为单分散球形颗粒，平均直径为10.47±1.80nm，晶格条纹间距为0.19nm。II型等温线和布鲁诺尔-埃米特-泰勒分析表明Ag-MOF-D具有介孔结构，表面积为5.3005 m²/g，平均孔径为9.46nm。对多药耐药细菌菌株的最低抑菌浓度和最低杀菌浓度值分别为3.90至7.80μM和7.8至62.5μM。对Vero细胞系的细胞毒性测试表明细胞活力呈剂量依赖性下降，IC值为1.701×10 mg/mL。这些发现表明Ag-MOF-D在抗菌应用和生物相容性方面具有潜力，在环境和食品安全应用方面具有未来机遇。
