（6-4）光产物从紫外线照射的哺乳动物细胞DNA中去除的效率比环丁烷嘧啶二聚体更高。
(6-4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers.
作者信息
Mitchell D L, Haipek C A, Clarkson J M
出版信息
Mutat Res. 1985 Jul;143(3):109-12. doi: 10.1016/s0165-7992(85)80018-x.
PMID:4010689
Abstract
A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.
摘要
一种针对紫外线照射的DNA产生的多克隆抗血清，可通过改变32P标记抗原的性质，特异性地检测环丁烷嘧啶二聚体和Pyr(6-4)Pyo光产物。在紫外线照射的人、仓鼠和小鼠细胞中，Pyr(6-4)Pyo光产物比环丁烷二聚体去除得更快。来自互补组A、C和D的着色性干皮病细胞在修复这两种损伤方面存在缺陷。
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