Zaba Melese Yilma, Neway Sebsib, Farrell Damien, Denion Eva, Perets Viktor, Tilahun Melaku, Bobosha Kidist, Cassidy Joseph P, Asmare Asefa, Gordon Stephen V
School of Veterinary Medicine, Wolaita Sodo University, Wolaita, Ethiopia.
School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
Access Microbiol. 2025 Mar 18;7(3). doi: 10.1099/acmi.0.000915.v3. eCollection 2025.
Bovine tuberculosis (bTB), caused by and other members of the complex (MTBC), is a significant concern for livestock and public health in Ethiopia. This study aimed to isolate and genetically characterize the causative agents of bTB in cattle from four abattoirs in the Wolaita zone of Ethiopia. A total of 2,251 cattle were examined post-mortem, and suspect tuberculous lesions were identified in 122 animals. From these animals, 180 tissue samples were collected and processed for bacteriological culture and genetic analysis, including the Loopamp commercial loop-mediated isothermal amplification kit, PCR targeting RD4 and RD9 loci and whole-genome sequencing (WGS). Bacteriological culture using mycobacteria growth indicator tube and Lowenstein-Jensen media ultimately identified 18 culture-positive samples, with WGS confirming in lesions from four animals and in lesions from one animal. The and isolates showed genetic similarity to previously identified MTBC lineages in Ethiopia. The presence of in cattle raises concerns about human-to-animal transmission. Additionally, non-tuberculous mycobacteria were isolated from lesions from multiple animals. Our study genetically characterized bacteria from suspect tuberculous lesions and provides the research community with new genome data for Ethiopian isolates of and .
牛结核病（bTB）由结核分枝杆菌复合群（MTBC）的结核分枝杆菌及其他成员引起，是埃塞俄比亚畜牧业和公共卫生的重大关切问题。本研究旨在从埃塞俄比亚沃莱塔地区四个屠宰场的牛中分离出牛结核病的病原体并进行基因特征分析。共对2251头牛进行了死后检查，在122头动物中发现了疑似结核病变。从这些动物身上采集了180份组织样本，进行细菌培养和基因分析，包括使用Loopamp商业环介导等温扩增试剂盒、针对RD4和RD9位点的PCR以及全基因组测序（WGS）。使用分枝杆菌生长指示管和罗氏培养基进行细菌培养最终鉴定出18个培养阳性样本，WGS在4头动物的病变中确认了结核分枝杆菌，在1头动物的病变中确认了牛分枝杆菌。结核分枝杆菌和牛分枝杆菌分离株与埃塞俄比亚先前鉴定的MTBC谱系具有遗传相似性。牛中存在牛分枝杆菌引发了对人畜传播的担忧。此外，从多只动物的病变中分离出了非结核分枝杆菌。我们的研究对疑似结核病变中的细菌进行了基因特征分析，并为埃塞俄比亚结核分枝杆菌和牛分枝杆菌分离株的研究界提供了新的基因组数据。
