Ustünes L, Claeys M, Laekeman G, Herman A G, Vlietinck A J, Ozer A
Prostaglandins. 1985 May;29(5):847-65. doi: 10.1016/0090-6980(85)90142-x.
Two fractions with prostaglandin E-like activity were isolated from onion (Allium cepa) by using XAD-2 adsorption, silicic acid column chromatography and thin layer chromatography. The fractions were analyzed by gas chromatography/mass spectrometry and were characterized as isomeric mixtures of 9,10,13-trihydroxy-11-octadecenoic and 9,12,13-trihydroxy-10-octadecenoic acid, which are lipoxygenase metabolites of linoleic acid. Bio-assay, for which cascade superfusion was used and the rabbit coeliac and mesenteric arteries and the rat fundus strip were employed as assay organs, was utilized to monitor the bio-active profile throughout the isolation procedures. The activity of 1 microgram of the pharmacologically active fractions T1 and T2 was found to be equivalent to that of respectively 1.33 and 0.63 ng of prostaglandin E2.
采用XAD - 2吸附、硅酸柱色谱和薄层色谱法从洋葱（葱属植物）中分离出两种具有前列腺素E样活性的组分。通过气相色谱/质谱联用仪对这些组分进行分析，其特征为9,10,13 - 三羟基 - 11 - 十八碳烯酸和9,12,13 - 三羟基 - 10 - 十八碳烯酸的异构体混合物，它们是亚油酸的脂氧合酶代谢产物。在整个分离过程中，采用级联灌注法，并以兔腹腔和肠系膜动脉以及大鼠胃底条带作为检测器官进行生物测定，以监测生物活性谱。发现1微克药理活性组分T1和T2的活性分别相当于1.33纳克和0.63纳克前列腺素E2的活性。
