Absalan Faezeh, Mehdipour Fereshteh, Shariat Mahmoud, Talei Abdoul-Rasoul, Ghaderi Abbas
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.
Department of Pathology, Shiraz Central Hospital Shiraz, Iran.
Am J Clin Exp Immunol. 2025 Feb 25;14(1):34-42. doi: 10.62347/VYQY9361. eCollection 2025.
IL-10 plays a crucial role in regulating B cell function and differentiation, but its effects on B cells depend on its activation state. The frequency of IL-10R B cells and how it changes during breast cancer progression have not been studied. This study aimed to evaluate the expression of IL-10R on B cells in the peripheral blood of 50 patients with breast cancer compared to 29 healthy controls. After isolating mononuclear cells, we stained them using anti-CD19 and anti-IL-10R antibodies. We used Flow cytometry to analyze the expression of IL-10R on B cells. We found that over 50% of B cells in both patients with breast cancer and healthy controls expressed IL-10R. However, patients had a significantly lower frequency of IL-10R B cells compared to healthy individuals (64±16.0% of patients compared to 78.5±6.6% for healthy individuals, P<0.0001). This decrease was not associated with lymph node involvement or tumor size. Phenotypic analysis revealed that IL-10R-expressing B cells consist of both naive and memory B cells, with the majority of peripheral memory B cells expressing IL-10R. The decrease in IL-10R-expressing B cells in breast cancer patients may be due to apoptosis induced by IL-10 in naive or recently activated B cells, or their migration to lymph nodes to combat tumor cells. Our study suggests that IL-10R could be a potential biomarker for detecting breast cancer in its early stages.
白细胞介素-10（IL-10）在调节B细胞功能和分化中起关键作用，但其对B细胞的影响取决于其激活状态。IL-10受体（IL-10R）阳性B细胞的频率及其在乳腺癌进展过程中的变化尚未得到研究。本研究旨在评估50例乳腺癌患者外周血B细胞中IL-10R的表达，并与29名健康对照进行比较。分离单核细胞后，我们使用抗CD19和抗IL-10R抗体对其进行染色。我们使用流式细胞术分析B细胞上IL-10R的表达。我们发现，乳腺癌患者和健康对照中超过50%的B细胞表达IL-10R。然而，与健康个体相比，患者的IL-10R阳性B细胞频率显著降低（患者为64±16.0%，健康个体为78.5±6.6%，P<0.0001）。这种降低与淋巴结受累或肿瘤大小无关。表型分析显示，表达IL-10R的B细胞包括幼稚B细胞和记忆B细胞，大多数外周记忆B细胞表达IL-10R。乳腺癌患者中表达IL-10R的B细胞减少可能是由于幼稚或近期激活的B细胞中IL-10诱导的细胞凋亡，或者它们迁移到淋巴结以对抗肿瘤细胞。我们的研究表明，IL-10R可能是早期检测乳腺癌的潜在生物标志物。
