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在变形链球菌OMZ 176的生长培养基中添加木糖醇——对可提取甘油磷酸聚合物合成的影响

Addition of xylitol to the growth medium of Streptococcus mutans OMZ 176--effect on the synthesis of extractable glycerol-phosphate polymers.

作者信息

Assev S, Vegarud G, Rölla G

出版信息

Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):145-9. doi: 10.1111/j.1699-0463.1985.tb02866.x.

DOI:10.1111/j.1699-0463.1985.tb02866.x
PMID:4013741
Abstract

Addition of 5% xylitol to growing cultures of Streptococcus mutans OMZ 176 reduced the amount of extractable glycerol-phosphate polymers in these cells compared to S. mutans cultures grown in medium with 5% glucose added. The glycerol-phosphate polymers were extracted from the cells by hot phenol-water-extraction, and separated by column chromatography. Lipoteichoic acid (LTA) was identified by indirect haemagglutination tests and lipid analysis. The amount of LTA extracted from the cells or present in the medium was not significantly different. It is suggested that the accumulation of intracellular xylitol-phosphate observed in a previous study causes an effect similar to glucose starvation, which is known to affect the composition of the cell wall.

摘要

与添加5%葡萄糖的培养基中生长的变形链球菌培养物相比,向变形链球菌OMZ 176的生长培养物中添加5%木糖醇会减少这些细胞中可提取的甘油磷酸聚合物的量。甘油磷酸聚合物通过热酚水提取法从细胞中提取出来,并通过柱色谱法进行分离。通过间接血凝试验和脂质分析鉴定脂磷壁酸(LTA)。从细胞中提取的或存在于培养基中的LTA量没有显著差异。有人提出,在先前的一项研究中观察到的细胞内木糖醇磷酸的积累会产生类似于葡萄糖饥饿的效应,已知葡萄糖饥饿会影响细胞壁的组成。

相似文献

1
Addition of xylitol to the growth medium of Streptococcus mutans OMZ 176--effect on the synthesis of extractable glycerol-phosphate polymers.在变形链球菌OMZ 176的生长培养基中添加木糖醇——对可提取甘油磷酸聚合物合成的影响
Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):145-9. doi: 10.1111/j.1699-0463.1985.tb02866.x.
2
Effect of growth conditions on the formation of extracellular lipoteichoic acid by Streptococcus mutans BHT.生长条件对变形链球菌BHT细胞外脂磷壁酸形成的影响。
Infect Immun. 1979 Jul;25(1):75-84. doi: 10.1128/iai.25.1.75-84.1979.
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Mode of elongation of the glycerol phosphate polymer of membrane lipoteichoic acid of Streptococcus faecium ATCC 9790.粪肠球菌ATCC 9790膜脂磷壁酸甘油磷酸聚合物的延伸模式。
J Bacteriol. 1981 Jul;147(1):75-9. doi: 10.1128/jb.147.1.75-79.1981.
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Further studies on the growth inhibition of Streptococcus mutans OMZ 176 by xylitol.木糖醇对变形链球菌OMZ 176生长抑制作用的进一步研究。
Acta Pathol Microbiol Immunol Scand B. 1986 Apr;94(2):97-102. doi: 10.1111/j.1699-0463.1986.tb03026.x.
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Evidence for presence of a xylitol phosphotransferase system in Streptococcus mutans OMZ 176.变形链球菌OMZ 176中存在木糖醇磷酸转移酶系统的证据。
Acta Pathol Microbiol Immunol Scand B. 1984 Apr;92(2):89-92. doi: 10.1111/j.1699-0463.1984.tb02800.x.
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Studies on the antibacterial activity of dodecylglycerol. Its limited metabolism and inhibition of glycerolipid and lipoteichoic acid biosynthesis in Streptococcus mutans BHT.十二烷基甘油的抗菌活性研究。其在变形链球菌BHT中的有限代谢以及对甘油脂和脂磷壁酸生物合成的抑制作用。
J Biol Chem. 1986 May 15;261(14):6338-45.
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Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture.果糖及其他碳水化合物对在连续培养中生长的变形链球菌英布里特株表面特性、脂磷壁酸产生及细胞外蛋白的影响。
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Effect of monoclonal antibodies against lipoteichoic acid from the oral bacterium Streptococcus mutans on its adhesion and plaque-accumulation in vitro.抗口腔细菌变形链球菌脂磷壁酸单克隆抗体对其体外黏附及菌斑聚集的影响
Arch Oral Biol. 1986;31(7):455-61. doi: 10.1016/0003-9969(86)90019-1.
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Effects of xylitol on the acid production activity from sorbitol by Streptococcus mutans and human dental plaque.木糖醇对变形链球菌和人牙菌斑利用山梨醇产酸活性的影响。
Swed Dent J. 1983;7(4):153-60.
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Effect of carbohydrate source and growth conditions on the production of lipoteichoic acid by Streptococcus mutans Ingbritt.碳水化合物来源及生长条件对变形链球菌英布里特株产脂磷壁酸的影响。
Infect Immun. 1979 Dec;26(3):1079-87. doi: 10.1128/iai.26.3.1079-1087.1979.

引用本文的文献

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Carbohydrate-controlled precipitation of apatite with coprecipitation of organic molecules in human saliva: stabilizing role of polyols.人唾液中碳水化合物控制的磷灰石沉淀及有机分子共沉淀:多元醇的稳定作用
Calcif Tissue Int. 1989 Apr;44(4):258-68. doi: 10.1007/BF02553760.