通过速率区带超速离心法分离出的青少年结缔组织病患者中的复合自身抗体。

Complexed autoantibodies in patients with juvenile connective tissue diseases, isolated by rate-zonal ultracentrifugation.

作者信息

Egeskjold E M, Johansen A, Høyeraal H M, Permin H, Johnsen V, Høier-Madsen M

出版信息

Acta Pathol Microbiol Immunol Scand C. 1985 Apr;93(2):71-7. doi: 10.1111/j.1699-0463.1985.tb02925.x.

DOI:10.1111/j.1699-0463.1985.tb02925.x

Abstract

Selected sera from one patient with systemic lupus erythematosus, two with mixed connective tissue disease, one with dermatomyositis, one with progressive systemic sclerosis and one with juvenile rheumatoid arthritis were investigated for autoantibodies after fractionation by computerized rate-zonal ultracentrifugation. Anti-Smith antibodies sedimented in an area from 6-11 S and anti-ribonucleoprotein from 6-13 S. IgG anti-IgG and IgG antinuclear antibodies (ANA) were present in free or complexed form in the 6-13 S area. IgM ANA occurred as 7 S IgM in patients with systemic lupus erythematosus and mixed connective tissue disease, whereas IgM ANA sedimented in the 19 S area in patients with dermatomyositis and progressive systemic sclerosis. Complexes containing IgG anti-IgG and ANA, positioned in the 6-13 S area are likely to play a significant role in the pathogenesis of systemic lupus erythematosus and mixed connective tissue disease.

摘要

选取一名系统性红斑狼疮患者、两名混合性结缔组织病患者、一名皮肌炎患者、一名进行性系统性硬化症患者和一名青少年类风湿性关节炎患者的血清，经计算机控制的速率区带超速离心分离后，检测其中的自身抗体。抗史密斯抗体沉降于6 - 11 S区域，抗核糖核蛋白抗体沉降于6 - 13 S区域。IgG抗IgG和IgG抗核抗体（ANA）以游离或复合形式存在于6 - 13 S区域。系统性红斑狼疮和混合性结缔组织病患者的IgM ANA以7 S IgM形式出现，而皮肌炎和进行性系统性硬化症患者的IgM ANA沉降于19 S区域。位于6 - 13 S区域的含有IgG抗IgG和ANA的复合物可能在系统性红斑狼疮和混合性结缔组织病的发病机制中起重要作用。