Beyan Ayşe Coşkun, Emerce Esra, Tuna Gamze, İşlekel Gül Hüray
Faculty of Medicine, Department of Occupational Medicine, Dokuz Eylul University, 35330 Izmir, Turkey.
Department of Toxicology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
Toxics. 2025 Mar 6;13(3):189. doi: 10.3390/toxics13030189.
Background/Objectives Using genotoxicity tests and oxidative stress indicators, the study evaluated the relationship between healthcare workers' (HCWs) exposure to chemical risks. This study aimed to evaluate the oxidative damage and genotoxic effects of sub chronic or long-term volatile organic compounds (VOCs) exposure in HCWs.
Pathology workers (Group 1), cleaning workers (Group 2), and medical secretaries (Group 3) were categorized, and VOCs and alkaline dust were measured for Groups 1 and 2 using appropriate occupational hygiene methods. Genotoxicity was assessed using alkaline comet and micronucleus (MN) assays. Oxidative stress indicators were analyzed in first-morning urine samples through liquid chromatography.
A total of 90 HCWs participated in the study. The mean R-cdA levels were 0.05 ± 0.02 for medical secretaries, 0.07 ± 0.03 for cleaning workers, and 0.06 ± 0.07 nmol/mmol creatinine for pathology workers ( = 0.040). The mean tail intensity (%) was 16.33 ± 10.68 (Group 1), 18.9 ± 7.4 for cleaning workers, and 14.1 ± 6.5 for medical secretaries ( = 0.020).
Implementing occupational hygiene measures in the working environment has effectively reduced occupational risks. The lack of significant differences in genotoxicity and oxidative stress parameters between the exposed and control groups supports the notion that the exposure limit values are protective.
背景/目的 本研究使用遗传毒性测试和氧化应激指标，评估医护人员（HCWs）接触化学风险之间的关系。本研究旨在评估医护人员亚慢性或长期接触挥发性有机化合物（VOCs）的氧化损伤和遗传毒性作用。
对病理科工作人员（第1组）、清洁工人（第2组）和医疗秘书（第3组）进行分类，使用适当的职业卫生方法对第1组和第2组测量VOCs和碱性粉尘。使用碱性彗星试验和微核（MN）试验评估遗传毒性。通过液相色谱分析晨尿样本中的氧化应激指标。
共有90名医护人员参与研究。医疗秘书的平均R-cdA水平为0.05±0.02，清洁工人为0.07±0.03，病理科工作人员为0.06±0.07 nmol/mmol肌酐（P = 0.040）。平均尾强度（%）在第1组为16.33±10.68，清洁工人为18.9±7.4，医疗秘书为14.1±6.5（P = 0.020）。
在工作环境中实施职业卫生措施可有效降低职业风险。暴露组和对照组之间遗传毒性和氧化应激参数无显著差异，这支持了暴露限值具有保护作用的观点。
