Castello Annamaria, Alio Vincenzina, Torresi Marina, Centorotola Gabriella, Chiaverini Alexandra, Pomilio Francesco, Arrigo Ignazio, Giammanco Anna, Fasciana Teresa, Ortoffi Marco Francesco, Gattuso Antonietta, Oliveri Giuseppa, Cardamone Cinzia, Costa Antonella
IZSSI-Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Via Gino Marinuzzi 3, 90129 Palermo, Italy.
IZSAM-Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Via Campo Boario, 64100 Teramo, Italy.
Pathogens. 2025 Mar 18;14(3):294. doi: 10.3390/pathogens14030294.
is an important foodborne pathogen, markedly persistent even in harsh environments and responsible for high hospitalization and mortality rates. The aim of the present study was to detect the strains circulating in Sicily over a five-year period and characterize their antimicrobial resistance profiles. The key element of this study was the sharing of data among various entities involved in food control and clinical surveillance of listeriosis in order to develop an integrated approach for this pathogen. A total of 128 isolates were analyzed, including 87 food-source strains and 41 clinical specimens. Whole-genome sequencing (WGS) was performed for sequence type (ST) and clonal complex (CC) identification through multilocus sequence typing (MLST) analysis. Antimicrobial resistance was assessed using the Kirby-Bauer method. The majority of strains belonged to serotype IVb (34/41 and 53/87 of clinical and food-source isolates, respectively) and were subtyped as CC2-ST2 (28/34 and 41/53 of clinical and food-source isolates respectively). Most of the isolates were susceptible to the main antimicrobials recommended for treatment of listeriosis. Resistance (R) and intermediate resistance (I) percentages worthy of attention were found against oxacillin (R: 85.9%) and clindamycin (I: 34.6%) in the food-source isolates and trimethoprim/sulfamethoxazole (R: 29.23%) in the clinical isolates. Also, 7.7% of the food-source isolates were multidrug resistant. Our results highlight how the punctual comparison between food and clinical strains is an essential tool for effectively tracking and preventing foodborne outbreaks.
是一种重要的食源性病原体，即使在恶劣环境中也具有显著的持久性，并且导致高住院率和死亡率。本研究的目的是检测西西里岛五年期间流行的菌株，并对其抗菌药物耐药谱进行表征。本研究的关键要素是参与李斯特菌病食品控制和临床监测的各个实体之间的数据共享，以便为这种病原体制定综合方法。共分析了128株分离株，包括87株食物源菌株和41份临床标本。通过多位点序列分型（MLST）分析进行全基因组测序（WGS）以鉴定序列类型（ST）和克隆复合体（CC）。使用 Kirby-Bauer 方法评估抗菌药物耐药性。大多数菌株属于血清型IVb（临床和食物源分离株分别为34/41和53/87），并被亚型化为CC2-ST2（临床和食物源分离株分别为28/34和41/53）。大多数分离株对推荐用于治疗李斯特菌病的主要抗菌药物敏感。在食物源分离株中，发现对苯唑西林（R：85.9%）和克林霉素（I：34.6%）以及临床分离株中对甲氧苄啶/磺胺甲恶唑（R：29.23%）的耐药（R）和中介耐药（I）百分比值得关注。此外，7.7%的食物源分离株对多种药物耐药。我们的结果凸显了食物菌株与临床菌株之间的及时比较是有效追踪和预防食源性疾病暴发的重要工具。
