Chaicumpa W, Ruangkunaporn Y, Burr D, Chongsa-Nguan M, Echeverria P
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J Clin Microbiol. 1992 Sep;30(9):2513-5. doi: 10.1128/jcm.30.9.2513-2515.1992.
A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Salmonella typhi was isolated from hemocultures, 73% in patients in whom S. typhi was isolated from stool specimens, and 40% in patients in whom the organism was isolated from bone marrow cultures. Among patients in whom S. typhi was isolated from blood cultures, the ELISA had a sensitivity of 65% when a single urine specimen was examined and 95% when serially collected urine specimens were examined. A dot blot immunoassay performed on a nitrocellulose filter in parallel had a sensitivity of 85%, versus 83% for the plate ELISA in which S. typhi was isolated from blood, bone marrow, and/or stool specimens. Since S. typhi antigen is intermittently excreted in the urine of patients with typhoid fever, serially collected urine from patients with typhoid should be tested for antigen 9.
一种针对D群沙门氏菌抗原9的单克隆抗体被用于间接酶联免疫吸附测定（ELISA），以检测从印度尼西亚雅加达临床伤寒热患者收集的尿液标本中的抗原。该ELISA在鉴定从血培养中分离出伤寒沙门氏菌的患者时灵敏度为95%，在从粪便标本中分离出伤寒沙门氏菌的患者中灵敏度为73%，在从骨髓培养物中分离出该菌的患者中灵敏度为40%。在从血培养中分离出伤寒沙门氏菌的患者中，检测单个尿液标本时ELISA的灵敏度为65%，检测连续收集的尿液标本时灵敏度为95%。同时在硝酸纤维素滤膜上进行的斑点印迹免疫测定的灵敏度为85%，而从血、骨髓和/或粪便标本中分离出伤寒沙门氏菌的平板ELISA的灵敏度为83%。由于伤寒沙门氏菌抗原在伤寒热患者尿液中呈间歇性排泄，因此应对伤寒患者连续收集的尿液进行抗原9检测。
