Huang Zhenbin, Wang Xia, Fan Liangshuai, Jin Xiaojun, Zhang Xiang, Wang Hongyan
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory of Arid Habitat Crops, Gansu Agricultural University, Lanzhou 730070, China.
Life (Basel). 2025 Mar 4;15(3):404. doi: 10.3390/life15030404.
Continuous cropping obstacles pose significant constraints and urgent challenges in the production of L. This experiment investigated the effects of consecutive cropping on over periods of 1, 2, and 3 years. It assessed the yield and quality of flower buds, in addition to the physicochemical properties of the rhizosphere soil. The microbial community in the rhizosphere was analyzed through 16S rDNA and ITS sequencing using Illumina Novaseq high-throughput sequencing technology, while also examining the correlations among these factors. The results reveal that as the duration of continuous cropping increases, the yield of flower buds, along with the contents of extract, tussilagone, and total flavonoids, steadily decreased; soil pH, organic matter, available phosphorus, available potassium, alkaline nitrogen, and the activities of sucrose, catalase, and alkaline phosphatase markedly decreased. As the duration of consecutive cropping increases, the quantity and diversity of bacteria in the rhizosphere soil initially increase and then decrease, while the number of fungal species increases by 22.5%. Meanwhile, continuous cropping of contributes to a gradual reduction in the relative abundance of beneficial genera such as Ralstonia, Nitrospira, and Trichoderma in the rhizosphere soil, while harmful genera such as Mortierella, Fusarium, and Tricharina accumulate significantly. Correlation analysis shows that changes in microbial communities notably influence the growth of and soil quality. This study elucidates the impacts of continuous cropping on the yield and quality of flower buds, soil physicochemical properties, and the microbial communities in the rhizosphere, providing a scientific basis for further research on continuous cropping barriers and the selection of beneficial microbial genera for the growth of .
连作障碍对L的生产构成了重大限制并带来了紧迫挑战。本实验研究了连作对L长达1年、2年和3年的影响。除了根际土壤的理化性质外，还评估了L花芽的产量和品质。采用Illumina Novaseq高通量测序技术，通过16S rDNA和ITS测序分析根际微生物群落，同时考察这些因素之间的相关性。结果表明，随着连作时间的增加，L花芽产量以及提取物、款冬酮和总黄酮含量稳步下降；土壤pH值、有机质、有效磷、有效钾、碱解氮以及蔗糖、过氧化氢酶和碱性磷酸酶的活性显著降低。随着连作时间的增加，根际土壤中细菌的数量和多样性先增加后减少，而真菌种类数量增加了22.5%。同时，L连作导致根际土壤中诸如罗尔斯通氏菌属、硝化螺旋菌属和木霉属等有益属的相对丰度逐渐降低，而诸如被孢霉属、镰刀菌属和木霉链菌属等有害属显著积累。相关性分析表明，微生物群落的变化显著影响L的生长和土壤质量。本研究阐明了连作对L花芽产量和品质、土壤理化性质以及根际微生物群落的影响，为进一步研究连作障碍及筛选有利于L生长的有益微生物属提供了科学依据。
