Bae Il Kwon, Park Yon-Koung, Park So Hyun, Hong Jun Sung
Department of Companion Animal Health and Sciences, Silla University, Busan 46958, Republic of Korea.
Microbiology Division, Busan Metropolitan City Institute of Health and Environment, Busan 46616, Republic of Korea.
Life (Basel). 2025 Mar 5;15(3):405. doi: 10.3390/life15030405.
Geckos are often considered to be reservoirs of zoonotic pathogens. This study was conducted to describe the prevalence and characteristics of pathogens isolated from fecal samples of crested geckos in South Korea. A total of 76 fecal samples were collected from 76 domesticated crested geckos in independent captivity. To determine bacterial profiles, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the disk diffusion method, PCR and direct sequencing, and the Kauffmann-White scheme for serotyping species were performed. A total of 107 Gram-negative isolates were identified as belonging to 50 species, 33 enterica subsp., 8 , 8 species, 3 , 2 , 2 , and 1 species. Most of the isolates were susceptible to antibiotics tested in this study. The chloramphenicol acetyltransferase (cat) gene was detected in one isolate, and the class C beta-lactamase (AZECL-14) gene was detected in one . The most prevalent somatic (O) antigens of the groups were C (n = 23) and D (n = 7), and 8 different serotypes were identified among the 33 enterica subsp. isolates. Five of eight serotypes have not been previously reported among clinical isolates in South Korea. Our results reveal that enteric bacteria have not been shared between crested geckos and humans, at least in South Korea.
壁虎常被认为是人畜共患病原体的宿主。本研究旨在描述从韩国有冠壁虎粪便样本中分离出的病原体的流行情况和特征。从76只单独圈养的家养有冠壁虎中总共收集了76份粪便样本。为了确定细菌谱，采用了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）、纸片扩散法、PCR和直接测序，以及考夫曼-怀特血清分型方案对菌种进行鉴定。总共鉴定出107株革兰氏阴性菌，分别属于50个菌种、33个肠炎亚种、8个其他菌种、8个其他菌种、3个其他菌种、2个其他菌种、2个其他菌种和1个其他菌种。本研究中测试的大多数分离株对所检测的抗生素敏感。在一株分离株中检测到氯霉素乙酰转移酶（cat）基因，在一株分离株中检测到C类β-内酰胺酶（AZECL-14）基因。这些菌群中最常见的菌体（O）抗原是C（n = 23）和D（n = 7），在33个肠炎亚种分离株中鉴定出8种不同的血清型。在韩国临床分离株中，8种血清型中有5种此前未见报道。我们的结果表明，至少在韩国，有冠壁虎和人类之间没有共享肠道细菌。
