Jiang Xin-Ru, Dai Ying-Yu, Wang Yu-Rong, Guo Kun, Du Yu, Gao Jian-Fang, Lin Long-Hui, Li Peng, Li Hong, Ji Xiang, Qu Yan-Fu
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China.
Animals (Basel). 2023 Apr 16;13(8):1365. doi: 10.3390/ani13081365.
Numerous studies have demonstrated that multiple intrinsic and extrinsic factors shape the structure and composition of gut microbiota in a host. The disorder of the gut microbiota may trigger various host diseases. Here, we collected fecal samples from wild-caught Japanese geckos () and captive conspecifics fed with mealworms (mealworm-fed geckos) and fruit flies (fly-fed geckos), aiming to examine the dietary and sexual correlates of the gut microbiota. We used 16S rRNA gene sequencing technology to determine the composition of the gut microbiota. The dominant phyla with a mean relative abundance higher than 10% were Verrucomicrobiota, Bacteroidota, and Firmicutes. Gut microbial community richness and diversity were higher in mealworm-fed geckos than in wild geckos. Neither community evenness nor beta diversity of gut microbiota differed among wild, mealworm-fed, and fly-fed geckos. The beta rather than alpha diversity of gut microbiota was sex dependent. Based on the relative abundance of gut bacteria and their gene functions, we concluded that gut microbiota contributed more significantly to the host's metabolic and immune functions. A higher diversity of gut microbiota in mealworm-fed geckos could result from higher chitin content in insects of the order Coleoptera. This study not only provides basic information about the gut microbiota of but also shows that gut microbiota correlates with dietary habits and sex in the species.
众多研究表明，多种内在和外在因素塑造了宿主肠道微生物群的结构和组成。肠道微生物群的紊乱可能引发宿主的各种疾病。在此，我们收集了野生捕获的日本壁虎（）以及喂食黄粉虫的圈养同种壁虎（喂食黄粉虫的壁虎）和喂食果蝇的圈养同种壁虎（喂食果蝇的壁虎）的粪便样本，旨在研究肠道微生物群与饮食及性别的相关性。我们使用16S rRNA基因测序技术来确定肠道微生物群的组成。平均相对丰度高于10%的优势菌门为疣微菌门、拟杆菌门和厚壁菌门。喂食黄粉虫的壁虎的肠道微生物群落丰富度和多样性高于野生壁虎。野生、喂食黄粉虫和喂食果蝇的壁虎之间，肠道微生物群的群落均匀度和β多样性均无差异。肠道微生物群的β多样性而非α多样性存在性别依赖性。基于肠道细菌的相对丰度及其基因功能，我们得出结论，肠道微生物群对宿主的代谢和免疫功能贡献更为显著。喂食黄粉虫的壁虎肠道微生物群多样性较高可能是由于鞘翅目昆虫中几丁质含量较高。本研究不仅提供了有关[物种名称]肠道微生物群的基础信息，还表明该物种的肠道微生物群与饮食习惯和性别相关。
