Sabu Priya, Pathak Harsh B, Nissen Emily, Chalise Prabhakar, Koestler Devin C, Godwin Andrew K, Umar Shahid, Spoozak Lori, Jewell Andrea, Mahoney Diane E
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Kansas Medical Center, USA.
University of Kansas Cancer Center, Kansas City, KS, USA.
Ann Obstet Gynecol. 2025;8(1). Epub 2025 Feb 12.
The role of the gut microbiome in non-gastrointestinal cancers has generated growing interest in the field of gynecologic oncology. Our objective was to characterize the gut microbiome in women with a pelvic mass suspicious for ovarian cancer. We hypothesized that (1) women with a pelvic mass would have reduced gut microbiota bacterial diversity compared to healthy controls and (2) gut microbial diversity would differ between benign disease compared to ovarian cancer.
In this case-control observational study, patients who presented with a suspicious pelvic mass were recruited from university affiliated gynecologic oncology clinics for fecal biospecimen donation. Fecal samples that were obtained from patients underwent 16S rRNA gene sequencing for microbial evaluation and statistical analysis. We used the Human Microbiome Project (HMP) Data Portal to compare gut microbiota profiles for our study to that of healthy female controls.
Fifteen patients with a pelvic mass were included ages 24-75 years. When comparing the gut microbiomes of these patients to 82 healthy females from the HMP Dataset, those with a pelvic mass had a significantly lower microbiota gut bacterial diversity. On the final pathology, 8 of the 15 patients with a suspicious pelvic mass had ovarian cancer and 7 had benign disease. Although not statistically significant, the alpha diversity was marginally reduced in patients with ovarian cancer compared to those with benign disease.
These findings underscore the necessity for validation in larger patient cohorts for clinical translation as a potential tool for disease diagnostics and disease prediction in diverse populations.
肠道微生物群在非胃肠道癌症中的作用在妇科肿瘤学领域引起了越来越多的关注。我们的目的是对盆腔肿块疑似卵巢癌的女性的肠道微生物群进行特征分析。我们假设：（1）与健康对照相比，盆腔肿块女性的肠道微生物群细菌多样性会降低；（2）良性疾病与卵巢癌患者的肠道微生物多样性会有所不同。
在这项病例对照观察性研究中，从大学附属妇科肿瘤诊所招募有可疑盆腔肿块的患者捐赠粪便生物样本。从患者处获得的粪便样本进行16S rRNA基因测序以进行微生物评估和统计分析。我们使用人类微生物组计划（HMP）数据门户将我们研究中的肠道微生物群概况与健康女性对照的概况进行比较。
纳入了15例盆腔肿块患者，年龄在24至75岁之间。将这些患者的肠道微生物群与HMP数据集中的82名健康女性进行比较时，有盆腔肿块的患者的微生物群肠道细菌多样性明显较低。在最终病理检查中，15例盆腔肿块可疑患者中有8例患有卵巢癌，7例患有良性疾病。虽然无统计学意义，但与良性疾病患者相比，卵巢癌患者的α多样性略有降低。
这些发现强调了在更大的患者队列中进行验证以实现临床转化的必要性，这是一种在不同人群中作为疾病诊断和疾病预测的潜在工具。
