Hu Weizhong, Zhou Shuxin, Ibrahim Amel, Li Guannan, Awad Sameh, Ramos-Vivas José, Kan Jianquan, Du Muying
College of Food Science, Southwest University, Chongqing 400715, China.
Chinese-Hungarian Cooperative Research Center for Food Science, Southwest University, Chongqing 400715, China.
Antibiotics (Basel). 2025 Mar 19;14(3):323. doi: 10.3390/antibiotics14030323.
As probiotics gain prominence in the prevention and treatment of intestinal diseases, their protective effects against pathogens and influence on host health have drawn significant attention. This study investigates the genomic characteristics and functional potential of XJ-24 (XJ-24) in the prevention of (LM) infection in mice. Whole-genome analysis confirmed the safety and probiotic properties of XJ-24, including acid and bile salt tolerance, antimicrobial activity, and safety. In vivo, C57BL/6 mice challenges indicated that XJ-24 significantly reduced LM colonization, suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), alleviated colon and spleen tissue damage, and maintained intestinal barrier integrity by upregulating tight junction proteins (Occludin, Claudin-1, ZO-1). Moreover, XJ-24 modulated gut microbiota composition by increasing beneficial taxa while reducing harmful bacteria. Correlation analysis highlighted a positive association between and tight junction proteins. These findings demonstrate the potential of XJ-24 as a functional probiotic for preventing LM infection and provide a basis for further clinical exploration.
随着益生菌在肠道疾病预防和治疗中的地位日益突出，它们对病原体的保护作用以及对宿主健康的影响已引起广泛关注。本研究调查了XJ-24在预防小鼠李斯特菌（LM）感染中的基因组特征和功能潜力。全基因组分析证实了XJ-24的安全性和益生菌特性，包括耐酸和耐胆盐能力、抗菌活性以及安全性。在体内，对C57BL/6小鼠的实验表明，XJ-24显著减少了LM的定植，抑制了促炎细胞因子（IL-1β、IL-6、TNF-α、IFN-γ），减轻了结肠和脾脏组织损伤，并通过上调紧密连接蛋白（闭合蛋白、Claudin-1、ZO-1）维持肠道屏障完整性。此外，XJ-24通过增加有益菌群同时减少有害细菌来调节肠道微生物群组成。相关性分析突出了[此处原文缺失相关内容]与紧密连接蛋白之间的正相关关系。这些发现证明了XJ-24作为预防LM感染的功能性益生菌的潜力，并为进一步的临床探索提供了依据。
