Chen Gongwei, Zhou Yixiao, Zhang Dandan, Chen Fengyuan, Qin Xuyang, Cai Hongyu, Gu Heng, Yue Yuanzheng, Wang Lianggui, Liu Guohua
School of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, No. 19 Wenchang East Road, Jurong 212400, China.
Key Laboratory of Landscape Architecture, College of Landscape Architecture, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China.
Genes (Basel). 2025 Mar 17;16(3):344. doi: 10.3390/genes16030344.
BACKGROUND/OBJECTIVES: The WRKY gene family plays a critical role in plant stress responses; however, its function in () under cold stress conditions remains poorly understood. This study aims to identify WRKY genes in , analyze their structural characteristics, and investigate their expression patterns under cold stress, thereby establishing a foundation for further exploration of their roles in cold stress responses.
Using transcriptional data from subjected to cold stress, we identified 46 WRKY family genes. We employed bioinformatics tools to conduct a comprehensive analysis of the physical and chemical properties of these genes, predict their subcellular localization, and construct a phylogenetic tree. A heatmap was generated to visualize the expression levels of WRKY genes across different treatment conditions. To validate our findings, qRT-PCR was performed on 10 highly expressed WRKY genes to analyze their temporal expression patterns during cold stress exposure.
The analysis revealed that WRKY genes in are predominantly localized to the nucleus, with protein lengths ranging from 55 to 1027 amino acids. Notably, all WRKY genes possessed the conserved WRKYGQK domain. Under cold stress conditions, the WRKY gene expression exhibited a general trend of increasing followed by decreasing, with peak expression observed at 24 h post-treatment. qRT-PCR analysis corroborated this pattern for the selected genes.
This study represents the first comprehensive structural and expression analysis of the WRKY gene family under cold stress conditions. Our findings provide valuable insights into their potential roles in plant cold stress responses, and lay the groundwork for future investigations into the molecular mechanisms underlying WRKY-mediated cold stress tolerance in .
背景/目的：WRKY基因家族在植物应激反应中起关键作用；然而，其在（）冷胁迫条件下的功能仍知之甚少。本研究旨在鉴定（）中的WRKY基因，分析其结构特征，并研究其在冷胁迫下的表达模式，从而为进一步探索它们在冷胁迫反应中的作用奠定基础。
利用（）冷胁迫下的转录数据，我们鉴定出46个WRKY家族基因。我们使用生物信息学工具对这些基因的理化性质进行了全面分析，预测了它们的亚细胞定位，并构建了系统发育树。生成了热图以可视化不同处理条件下WRKY基因的表达水平。为了验证我们的发现，对10个高表达的WRKY基因进行了qRT-PCR，以分析它们在冷胁迫暴露期间的时间表达模式。
分析表明，（）中的WRKY基因主要定位于细胞核，蛋白质长度从55到1027个氨基酸不等。值得注意的是，所有WRKY基因都具有保守的WRKYGQK结构域。在冷胁迫条件下，WRKY基因表达呈现先升高后降低的总体趋势，在处理后24小时观察到表达峰值。qRT-PCR分析证实了所选基因的这种模式。
本研究是首次在冷胁迫条件下对（）WRKY基因家族进行全面的结构和表达分析。我们的发现为它们在植物冷胁迫反应中的潜在作用提供了有价值的见解，并为未来研究（）中WRKY介导的冷胁迫耐受性的分子机制奠定了基础。
