从一种耐紫外线和伽马辐射的细菌中鉴定抗菌肽。

Identification of Antimicrobial Peptides from , a UV and Gamma Radiation Tolerant Bacterium.

作者信息

Kim Sam Woong, Bang Woo Young

机构信息

Agri-Food Bio Convergence Institute, Gyeongsang National University, Jinju 52725, Republic of Korea.

Biodiversity Research and Cooperation Division, National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 22689, Republic of Korea.

出版信息

Genes (Basel). 2025 Mar 19;16(3):353. doi: 10.3390/genes16030353.

DOI:10.3390/genes16030353

PMID:40149504

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11941926/

Abstract

BACKGROUND

, a UV and gamma radiation-tolerant bacterium, was reported to have superior antibacterial activities against a variety of pathogenic bacteria through the production of antimicrobial peptides (AMPs), but nothing is known about its AMPs.

METHODS/RESULTS: In this study, our genomic and transcriptomic data showed that the genome contains 11 gene candidates, designated as NB_AMP1 to NB_AMP11, which are expressed differently in logarithmic growth and stationary phase. Moreover, the cell-free supernatant of all DH5α strains containing cloned except for NB_AMP5 and NB_AMP7 exhibited antibacterial activities against both Gram-negative and Gram-positive bacteria such as and . Synthetic AMPs supported the antibacterial activities of cloned , and, in particular, the synthetic NB_AMP2 showed superior antibacterial activities against both and .

CONCLUSIONS

Altogether, these results suggest that the candidates from may function as antimicrobial peptides, effectively causing cellular lysis through pore formation in the bacterial membrane.

摘要

背景

据报道，一种耐紫外线和伽马辐射的细菌通过产生抗菌肽（AMPs）对多种病原菌具有卓越的抗菌活性，但其抗菌肽情况未知。

方法/结果：在本研究中，我们的基因组和转录组数据显示，该细菌基因组含有11个候选基因，命名为NB_AMP1至NB_AMP11，它们在对数生长期和稳定期表达不同。此外，除NB_AMP5和NB_AMP7外，所有含有克隆该细菌的DH5α菌株的无细胞上清液对革兰氏阴性菌和革兰氏阳性菌如大肠杆菌和金黄色葡萄球菌均表现出抗菌活性。合成抗菌肽证实了克隆该细菌的抗菌活性，特别是合成的NB_AMP2对大肠杆菌和金黄色葡萄球菌均表现出卓越的抗菌活性。