Torinuki W, Miura T, Tagami H
Arch Dermatol Res. 1985;277(3):174-8. doi: 10.1007/BF00404312.
Sera from three patients with porphyria cutanea tarda (PCT) were examined for evidence of complement activation. The irradiation of sera in vitro with 405-nm light resulted in a dose-dependent diminution of total hemolytic complement activity and the hemolytic titers of C1, C4, C2, C3, and C5. Furthermore, such irradiated sera showed immunoelectrophoretical C3 conversion, chemotactic activity for rat polymorphonuclear leukocytes inhibited by incubation with anti-C5 antisera but not anti-C3 antisera, and C5a generation as measured by radioimmunoassay. Factor-B conversion did not occur in such irradiated sera. Using Sephadex G-75 chromatography, the irradiated sera showed a multiphasic elution profile of chemotactic activity similar to that of zymosan-activated serum. The generation of C5a even occurred in factor-B-depleted serum. These studies indicate that the irradiation of PCT serum with 405-nm light induces the activation of the complement system via the classical pathway, resulting in the development of a chemotactic factor.
对三名迟发性皮肤卟啉病（PCT）患者的血清进行了补体激活证据的检测。用405纳米光对血清进行体外照射导致总溶血补体活性以及C1、C4、C2、C3和C5的溶血效价呈剂量依赖性降低。此外，经这种照射的血清显示出免疫电泳C3转化、对大鼠多形核白细胞的趋化活性（与抗C5抗血清孵育可抑制，而与抗C3抗血清孵育则不能抑制）以及通过放射免疫测定法测得的C5a生成。在这种经照射的血清中未发生B因子转化。使用葡聚糖凝胶G - 75色谱法，经照射的血清显示出与酵母聚糖激活血清相似的趋化活性多相洗脱图谱。即使在B因子缺失的血清中也会发生C5a生成。这些研究表明，用405纳米光照射PCT血清会通过经典途径诱导补体系统激活，从而导致趋化因子的产生。
