Fernandes Beatriz Pasqualli, Girardini Lilian Kolling, Prestes Alan M, Kominkiewicz Marcelo, Maciel Julcimar Machado, da Costa Mateus Matiuzzi, Tavares Marcio Rennan Santos, da Motta Amanda de Souza
Institute of Health Science, Microbiology, Immunology and Parasitology Department, Federal University of Rio Grande do Sul, 222/500 Sarmento Leite Street, Porto Alegre 90050-170, Brazil.
Western University of Santa Catarina (UNOESC), Xanxerê 88040-900, Santa Catarina, Brazil.
ACS Omega. 2025 Mar 11;10(11):11525-11533. doi: 10.1021/acsomega.5c00190. eCollection 2025 Mar 25.
Evaluation of natural molecules for replacement of growth promoters applied in poultry has been studied, as it can provide a favorable intestinal environment to improve the digestion and absorption capacity of nutrients. Essential oils of oregano, clove, rosemary, and propionic, lactic, and formic organic acids were tested against 30 different species. The antimicrobial potential was tested, with formic acid having the highest mean of inhibition halos (32.09 ± 1.01 mm) and clove EO having an average of 14.36 ± 0.54 mm. The synergy between formic acid and clove was observed against Typhimurium (∑FIC 0.37), Enteritidis (∑FIC 0.07), and APEC (∑FIC 0.265). Through the in silico method, the potential of EO and OA to bind the proteins d-glutamate ligase (PDB ID: 1E0D) and DNA gyrase B (PDB ID: 4PRV) was evaluated. It was confirmed that the molecules with the highest binding affinity were formic acid and clove. The antioxidant power was evaluated by the DPPH free radical capture method, and the clove EO showed higher activity (IC: 3.029 μg mL). The results demonstrate that the products have antimicrobial and antioxidant properties, suggesting applicability in synergistic formulations, which may be effective for a wide variety of pathogens in poultry. The association between two natural molecules can be possible substitutes for growth promoters, with applications in feed formulations.
人们已经对用于替代家禽生长促进剂的天然分子进行了研究，因为它可以提供有利的肠道环境，以提高营养物质的消化和吸收能力。对牛至、丁香、迷迭香的精油以及丙酸、乳酸和甲酸等有机酸针对30种不同菌种进行了测试。测试了其抗菌潜力，其中甲酸的平均抑菌圈最大（32.09±1.01毫米），丁香精油的平均抑菌圈为14.36±０.54毫米。观察到甲酸和丁香对鼠伤寒沙门氏菌（∑FIC 0.37）、肠炎沙门氏菌（∑FIC 0.07）和禽致病性大肠杆菌（∑FIC 0.265）具有协同作用。通过计算机模拟方法，评估了精油和有机酸与蛋白质d-谷氨酸连接酶（PDB ID：1E0D）和DNA促旋酶B（PDB ID：4PRV）结合的潜力。已证实具有最高结合亲和力的分子是甲酸和丁香。通过DPPH自由基捕获法评估了抗氧化能力，丁香精油显示出更高的活性（IC：3.029微克/毫升）。结果表明，这些产品具有抗菌和抗氧化特性，表明其在协同配方中的适用性，这可能对家禽中的多种病原体有效。两种天然分子的组合可能是生长促进剂的替代品，可应用于饲料配方中。
