Leary A G, Strauss L C, Civin C I, Ogawa M
Blood. 1985 Aug;66(2):327-32.
We analyzed the differentiation of hemopoietic colonies derived from human paired daughter cells. Candidate progenitor cells were isolated by use of a micromanipulation technique from cultures of My-10 antigen-positive cord blood cells. Then nine to 36 hours later, the paired daughter cells were separated with a micromanipulator and allowed to form colonies in methylcellulose medium containing erythropoietin, phytohemagglutinin leukocyte-conditioned medium, and platelet-poor plasma. The cellular composition of the colonies was determined by differentiating all of the cells of the May-Grünwald-Giemsa-stained preparation. Of a total of 75 evaluable pairs of colonies, 35 consisted of 28 types of disparate pairs revealing nonhomologous lineage combinations. Forty pairs were homologous in lineage expression. However, the proportions of the individual cell lineages were significantly different in the members of some of the homologous pairs. Some pairs revealed significant differences in colony size. These observations are similar to those reported for murine paired progenitors and are consistent with the stochastic model of human stem cell differentiation.
我们分析了源自人类配对子细胞的造血集落的分化情况。通过显微操作技术从My-10抗原阳性脐血细胞培养物中分离候选祖细胞。然后在9至36小时后，用显微操作器分离配对的子细胞，并使其在含有促红细胞生成素、植物血凝素白细胞条件培养基和少血小板血浆的甲基纤维素培养基中形成集落。通过对May-Grünwald-Giemsa染色制剂的所有细胞进行分化来确定集落的细胞组成。在总共75对可评估的集落中，35对由28种不同类型的不同对组成，显示出非同源谱系组合。40对在谱系表达上是同源的。然而，在一些同源对的成员中，各个细胞谱系的比例存在显著差异。一些对在集落大小上显示出显著差异。这些观察结果与报道的小鼠配对祖细胞的观察结果相似，并且与人类干细胞分化的随机模型一致。
