Dickter Jana K, Zhao Yuqi, Parekh Vishwas, Ma Huiyan, Modi Badri G, Li Wai-Yee, Armenian Saro H, Wu Xiwei, Abdulla Farah R
Department of Medicine, Division of Infectious Diseases, City of Hope National Medical Center, Duarte, California, USA.
Beckman Research Institute, City of Hope National Medical Center, Duarte, California, USA.
Microbiol Spectr. 2025 Mar 31;13(5):e0005325. doi: 10.1128/spectrum.00053-25.
We investigated the presence of viral DNA and RNA in cutaneous squamous cell carcinoma (cSCC) tumor and normal tissues from nine individuals with a history of hematopoietic stem cell transplantation (HCT). Microbiome quantification through DNA and RNA sequencing (RNA-seq) revealed the presence of 18 viruses in both tumor and normal tissues. DNA sequencing (DNA-seq) identified , , , , , , and others. RNA-seq revealed additional viruses such , , , , , , , and others. Notably, DNA-seq indicated that tumor samples exhibited low levels of in three out of nine subjects and elevated levels of in one subject, while normal samples frequently contained and occasionally . A comparative analysis using both DNA- and RNA-seq captured three common viruses: , , and . These findings were corroborated by an independent data set, supporting the reliability of the viral detection methods utilized. The study provides insights into the viral landscape in post-HCT patients, emphasizing the need for comprehensive viral monitoring in this vulnerable population.
This study is important because it explores the potential role of viruses in the development of cSCC in individuals who have undergone allogeneic HCT. cSCC is common in this population, particularly in those with chronic graft-versus-host disease on long-term immunosuppression. By using advanced metagenomic and metatranscriptomic next-generation sequencing, we aimed to identify viral pathogens present in tumor and adjacent normal tissue. The results could lead to targeted preventive or therapeutic interventions for these high-risk people, potentially improving their outcomes and management of cSCC.
我们调查了9名有造血干细胞移植（HCT）病史的个体的皮肤鳞状细胞癌（cSCC）肿瘤组织和正常组织中病毒DNA和RNA的存在情况。通过DNA和RNA测序（RNA-seq）进行微生物群落定量分析，结果显示肿瘤组织和正常组织中均存在18种病毒。DNA测序（DNA-seq）鉴定出了……等。RNA-seq还揭示了其他病毒，如……等。值得注意的是，DNA-seq表明，在9名受试者中有3名的肿瘤样本中……水平较低，而在1名受试者中……水平升高，而正常样本中经常含有……，偶尔含有……。使用DNA-seq和RNA-seq的比较分析发现了三种常见病毒：……、……和……。这些发现得到了一个独立数据集的证实，支持了所使用的病毒检测方法的可靠性。该研究为HCT后患者的病毒情况提供了见解，强调了在这一脆弱人群中进行全面病毒监测的必要性。
这项研究很重要，因为它探讨了病毒在接受异基因HCT的个体发生cSCC过程中的潜在作用。cSCC在这一人群中很常见，尤其是那些长期接受免疫抑制治疗且患有慢性移植物抗宿主病的患者。通过使用先进的宏基因组和宏转录组下一代测序技术，我们旨在鉴定肿瘤组织和相邻正常组织中存在的病毒病原体。研究结果可能会为这些高危人群带来有针对性的预防或治疗干预措施，有可能改善他们的cSCC治疗效果和管理水平。
