International Agency for Research on Cancer-World Health Organization, Lyon, France; "Magna Graecia" University, Catanzaro, Italy.
International Agency for Research on Cancer-World Health Organization, Lyon, France.
Papillomavirus Res. 2020 Jun;9:100196. doi: 10.1016/j.pvr.2020.100196. Epub 2020 Mar 25.
Actinic keratosis (AK) arises on photo-damaged skin and is considered to be the precursor lesion of cutaneous squamous cell carcinoma (cSCC). Many findings support the involvement of β human papillomaviruses (HPVs) in cSCC, while very little is known on γ HPV types. The objective of this study was to characterize the spectrum of PV types in healthy skin (HS) and AK samples of the same immunocompetent individuals using next generation sequencing (NGS). Viral DNA of 244 AK and 242 HS specimens were amplified by PCR using two different sets of primers (FAP59/64 and FAPM1). Purified amplicons were pooled and sequenced using NGS. The study resulted in the identification of a large number of known β and γ PV types. In addition, 27 putative novel β and 16 γ and 4 unclassified PVs were isolated. HPV types of species γ-1 (e.g. HPV4) appeared to be strongly enriched in AK versus HS. The NGS analysis revealed that a large spectrum of known and novel PVs is present in HS and AK. The evidence that species γ-1 HPV types appears to be enriched in AK in comparison to HS warrants further studies to evaluate their role in development of skin (pre)cancerous lesions.
光化性角化病（AK）发生于光损伤皮肤，被认为是皮肤鳞状细胞癌（cSCC）的前体病变。许多研究结果支持β型人乳头瘤病毒（HPV）在 cSCC 中的作用，而关于 γ 型 HPV 类型的了解甚少。本研究旨在使用下一代测序（NGS）技术，对同一免疫功能正常个体的健康皮肤（HS）和 AK 样本中的 HPV 类型谱进行特征分析。使用两套不同的引物（FAP59/64 和 FAPM1）对 244 个 AK 和 242 个 HS 样本的病毒 DNA 进行 PCR 扩增。纯化的扩增子经 NGS 测序。该研究鉴定出了大量已知的 β 和 γ HPV 类型。此外，还分离出了 27 种新型 β 和 16 种 γ 和 4 种未分类的 HPV。与 HS 相比，属 γ-1（如 HPV4）的 HPV 类型似乎在 AK 中强烈富集。NGS 分析表明，在 HS 和 AK 中存在大量已知和新型的 HPV 类型。与 HS 相比，属 γ-1 HPV 类型在 AK 中似乎更丰富，这一证据值得进一步研究，以评估它们在皮肤（癌前）病变发展中的作用。
