Kang Liqin, Zhu Xinji, Yan Yangtian, Zhu Rui, Wei Wei, Peng Fei, Sun Lei
School of Life Sciences and Chemical Engineering, Jiangsu Second Normal University, Nanjing, China.
Department of Critical Care Medicine, School of Medicine, Southeast University, Nanjing, China.
Chem Biodivers. 2025 Aug;22(8):e202500120. doi: 10.1002/cbdv.202500120. Epub 2025 Apr 14.
In this present work, the polysaccharides from Agaricus bisporus were extracted and fractioned with gradient ethanol precipitation method for the first time. Five fractions (ABP40, ABP50, ABP60, ABP70, and ABP80) were obtained with ethanol concentrations of 40%, 50%, 60%, 70%, and 80%, respectively, and their characteristics and antioxidant activities in vitro were investigated. The five fractions presented significant differences in total sugar, protein, and uronic acid content, with a marked discrepancy in the molar ratio of the monosaccharide composition. The molecular weights of the polysaccharides decreased with increasing ethanol concentration. Compared to the other four fractions, ABP70, which has the highest uronic acid content, showed more conspicuous radical-scavenging activities against hydroxyl (89.9 ± 0.33%) and DPPH radicals (80.1 ± 0.01%). Moreover, it was found that the total sugar content and antioxidant activities of polysaccharides increased with the extension of precipitation time, with the highest antioxidant activities at 24 h. Therefore, ABP70, precipitated for 24 h, may have a potential application value for the development of antioxidants. This study provides valuable information for the further commercial applications of polysaccharides from Agaricus bisporus.
在本研究中，首次采用梯度乙醇沉淀法对双孢蘑菇多糖进行提取和分级分离。分别用浓度为40%、50%、60%、70%和80%的乙醇获得了五个级分（ABP40、ABP50、ABP60、ABP70和ABP80），并对其特性和体外抗氧化活性进行了研究。这五个级分在总糖、蛋白质和糖醛酸含量上存在显著差异，单糖组成的摩尔比也有明显差异。多糖的分子量随乙醇浓度的增加而降低。与其他四个级分相比，糖醛酸含量最高的ABP70对羟基自由基（89.9±0.33%）和DPPH自由基（80.1±0.01%）表现出更显著的清除活性。此外，还发现多糖的总糖含量和抗氧化活性随沉淀时间的延长而增加，在24小时时抗氧化活性最高。因此，沉淀24小时的ABP70在抗氧化剂开发方面可能具有潜在的应用价值。本研究为双孢蘑菇多糖的进一步商业应用提供了有价值的信息。
