Halder Ankit, Dutta Suhisna, Srivastava Sanjeeva
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India.
Methods Mol Biol. 2025;2914:183-199. doi: 10.1007/978-1-0716-4462-1_14.
The cerebrospinal fluid (CSF) plays a critical role in maintaining homeostasis within the central nervous system (CNS). Detecting protein alterations in CSF associated with brain tumors offers a unique opportunity to enhance diagnosis, prognosis, recurrence monitoring, and treatment response. As an extracellular component of the CNS, it serves as a valuable reservoir of biomarkers that reflect changes associated with brain tumors-biomarkers that are often difficult to detect in the systemic circulation due to the blood-brain barrier. Advances in liquid biopsy techniques for CSF, coupled with high-throughput technologies, have the potential to significantly benefit patients. Here, we provide a comprehensive overview of methodologies for studying the CSF proteome to identify brain tumor-associated alterations.
脑脊液（CSF）在维持中枢神经系统（CNS）内的稳态中起着关键作用。检测与脑肿瘤相关的脑脊液中的蛋白质变化为提高诊断、预后、复发监测和治疗反应提供了独特的机会。作为中枢神经系统的细胞外成分，它是反映与脑肿瘤相关变化的生物标志物的宝贵储存库——由于血脑屏障，这些生物标志物在体循环中往往难以检测到。脑脊液液体活检技术的进展，再加上高通量技术，有可能使患者显著受益。在此，我们全面概述了研究脑脊液蛋白质组以识别脑肿瘤相关变化的方法。
