Fei Yuzhi, Afzal Shah Faheem, Chen Zhu, Zhao Yue, Meng Xin, Ren Jie, Zhang Shuiming
Department of Ornamental Horticulture, School of Horticulture, Anhui Agricultural University, Hefei, 230036, Anhui, China.
Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
Genes Genomics. 2025 Apr 1. doi: 10.1007/s13258-025-01638-7.
The NAC family (consisting of NAM, ATAF1/2, and CUC2) represents a crucial plant-specific transcription factor family, contributing significantly to various aspects of plant growth, development, and reactions to abiotic stresses. Yet, the underlying mechanism of NAC regulation in Acer paxii has not been discussed.
Identification of NAC genes (ApNACs) in the genome of Acer paxii and exploring the regulatory network of NACs in mediating leaf senescence.
A thorough genome-wide analysis of the NAC gene family in the Acer paxii genome was performed.
We identified 117 ApNACs from the Acer paxii genome database, which were irregularly distributed across 13 chromosomes. Phylogenetic analysis revealed that ApNAC genes were partitioned into 16 subgroups. There are four kinds of cis-regulatory elements in the promoter region of the ApNACs gene. We compared the expression levels of ApNAC genes at different times using transcriptome data and selected six ApNAC genes for qRT-PCR, which the results showed basic consistency with the transcriptome results. Six ApNACs and 187 TFs from different families were selected, and it was found that the TF families with the highest correlation were WRKY, MYB, bZIP, and ERF, and these TF families were reported to participate in the regulation function in senescence.
This study provides important data support for identifying the NAC gene family of Acer paxii and the regulatory function of the ApNAC genes on plant senescence, which will help to understand the NAC-mediated regulatory network in Acer paxii.
NAC家族（由NAM、ATAF1/2和CUC2组成）是植物特有的关键转录因子家族，对植物生长、发育及非生物胁迫反应的多个方面有重要贡献。然而，NAC在青榨槭中的调控机制尚未见讨论。
鉴定青榨槭基因组中的NAC基因（ApNACs），并探索NACs在介导叶片衰老中的调控网络。
对青榨槭基因组中的NAC基因家族进行全面的全基因组分析。
我们从青榨槭基因组数据库中鉴定出117个ApNACs，它们不规则地分布在13条染色体上。系统发育分析表明，ApNAC基因被分为16个亚组。ApNACs基因启动子区域存在四种顺式调控元件。我们利用转录组数据比较了不同时间ApNAC基因的表达水平，并选择了6个ApNAC基因进行qRT-PCR，结果与转录组结果基本一致。选择了6个ApNACs和187个来自不同家族的转录因子，发现相关性最高的转录因子家族是WRKY、MYB、bZIP和ERF，并且据报道这些转录因子家族参与衰老调控功能。
本研究为鉴定青榨槭的NAC基因家族以及ApNAC基因对植物衰老的调控功能提供了重要的数据支持，这将有助于了解青榨槭中NAC介导的调控网络。
