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异色瓢虫的染色体水平基因组组装

Chromosome-level genome assembly of the Adonis ladybird Hippodamia variegata.

作者信息

Liu Hong-Ling, Yong Yan-Ping, Wu Xing-Long, Chen Zhi-Teng, Wei Shu-Jun, Cai Peng, Pu De-Qiang

机构信息

Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.

School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212004, China.

出版信息

Sci Data. 2025 Apr 1;12(1):558. doi: 10.1038/s41597-025-04882-4.

Abstract

The Adonis ladybird (Hippodamia variegata), an important predator in agricultural ecosystems, plays a crucial role in biological control and is a significant model for evolutionary and genomic studies within Coccinellidae. Despite its ecological importance, the lack of a reference genome for H. variegata has limited in-depth investigations into its biology and potential as a biocontrol agent. Here, we present a high-quality, chromosome-level genome assembly of H. variegata. The final assembly spans 493.01 Mb, with a scaffold N50 of 28.19 Mb and a GC content of 36.41%. Hi-C sequencing data enabled the anchoring of 343.20 Mb to 10 chromosomes. Repetitive elements accounted for 258.56 Mb (52.44%) of the genome, with long interspersed nuclear elements (LINEs) being the most prevalent. We identified 37,348 protein-coding genes, of which 78.55% were functionally annotated in public protein databases. This high-quality genome assembly will serve as a valuable resource for furthering our understanding of Adonis ladybird's evolutionary biology, enhancing its utility in pest management, and supporting future research on ladybird genomics.

摘要

阿多尼斯瓢虫(异色瓢虫)是农业生态系统中的一种重要捕食者,在生物防治中发挥着关键作用,并且是瓢虫科进化和基因组研究的重要模式生物。尽管其具有生态重要性,但由于缺乏异色瓢虫的参考基因组,对其生物学特性和作为生物防治剂的潜力的深入研究受到了限制。在此,我们展示了一个高质量的、染色体水平的异色瓢虫基因组组装。最终组装的基因组跨度为493.01 Mb,支架N50为28.19 Mb,GC含量为36.41%。Hi-C测序数据使343.20 Mb的序列能够定位到10条染色体上。重复元件占基因组的258.56 Mb(52.44%),其中长散在核元件(LINEs)最为普遍。我们鉴定出37348个蛋白质编码基因,其中78.55%在公共蛋白质数据库中得到了功能注释。这个高质量的基因组组装将成为一个有价值的资源,有助于我们进一步了解阿多尼斯瓢虫的进化生物学,提高其在害虫管理中的效用,并支持未来对瓢虫基因组学的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d081/11961608/72179249dc73/41597_2025_4882_Fig1_HTML.jpg

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