使用凝胶电泳分析核酸纳米结构的核酸酶降解。
Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis.
机构信息
The RNA Institute, University at Albany, State University of New York, Albany, New York.
出版信息
Curr Protoc Nucleic Acid Chem. 2020 Sep;82(1):e115. doi: 10.1002/cpnc.115.
Custom-built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such applications, the field faces a challenge in making biostable DNA nanostructures. Being a natural material, DNA is most suited for biological applications, but is also easily degraded by nucleases. Several methods have been employed to study the nuclease degradation rates and enhancement of nuclease resistance. This protocol describes the use of gel electrophoresis to analyze the extent of nuclease degradation of DNA nanostructures and to report degradation times, kinetics of nuclease digestion, and evaluation of biostability enhancement factors. © 2020 Wiley Periodicals LLC. Basic Protocol: Timed analysis of nuclease degradation of DNA nanostructures Support Protocol: Calculating biostability enhancement factors.
定制的 DNA 纳米结构现在被应用于生物传感、分子计算、生物分子分析和药物输送等领域。虽然 DNA 的功能和生物相容性使得 DNA 纳米结构在这些应用中很有用，但该领域在构建具有生物稳定性的 DNA 纳米结构方面面临挑战。作为一种天然材料，DNA 最适合用于生物应用，但也很容易被核酸酶降解。已经采用了几种方法来研究核酸酶的降解速率和增强核酸酶抗性。本协议描述了使用凝胶电泳分析 DNA 纳米结构的核酸酶降解程度，并报告降解时间、核酸酶消化动力学和生物稳定性增强因子的评估。© 2020Wiley Periodicals LLC. 基本方案：DNA 纳米结构的核酸酶降解时间分析 支持方案：计算生物稳定性增强因子。
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