Altattan Basma, Möser Christin, Smith David
Institute for Molecular Diagnostics und Bioanalysis (IMDB), Potsdam, Germany.
Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
Methods Mol Biol. 2025;2901:179-189. doi: 10.1007/978-1-0716-4394-5_14.
The provided protocol outlines a method for synthesizing a peptide-modified three-arm DNA nanostructure. The protocol details the functionalization of three partially complementary amino-modified single-stranded DNA molecules with azide-modified peptide, denoted "PeB," using copper-free click chemistry. This PeB peptide, derived from the neutralizing antibody HC19, effectively targets the influenza A virus. Subsequently, the peptide-functionalized DNA structures are assembled into a three-arm DNA structure, enabling multivalent presentation of the peptides to enhance binding and inhibit viral particles. While this protocol specifically describes the synthesis of simple trivalent DNA-peptide structures, the general approach can be scaled to decorate more complex DNA nanostructures such as those formed from tile- or origami-based architectures.
所提供的方案概述了一种合成肽修饰的三臂DNA纳米结构的方法。该方案详细介绍了使用无铜点击化学方法，用叠氮化物修饰的肽（称为“PeB”）对三个部分互补的氨基修饰单链DNA分子进行功能化。这种源自中和抗体HC19的PeB肽可有效靶向甲型流感病毒。随后，将肽功能化的DNA结构组装成三臂DNA结构，使肽能够多价呈现，从而增强结合并抑制病毒颗粒。虽然该方案具体描述了简单三价DNA-肽结构的合成，但一般方法可以扩展用于修饰更复杂的DNA纳米结构，如由基于瓦片或折纸的结构形成的那些。
