Han Qingyue, Yu Jingrong, Gao Zhengkun, Li Sitong, Wang Zi, Wu Jie, Huang Fengjie
College of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, PR China.
College of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, PR China.
Int J Biol Macromol. 2025 May;309(Pt 1):142738. doi: 10.1016/j.ijbiomac.2025.142738. Epub 2025 Apr 1.
Intestinal perforation is a serious medical emergency, and traditional surgery often causes adhesion and other complications. Innovative hydrogels improve postoperative care and rehabilitation with their anti-adhesion, antibacterial, and hemostatic properties. We have developed an advanced anti-adhesion hydrogel, AA-A30, composed of polypyrrole-modified gelatin (PPy-GelMA), carboxymethyl chitosan (CMCS), and NHS-functionalized polyethylene glycol (PEG-NHS). This hydrogel is specifically tailored for intestinal perforations. Upon hydrolysis, PEG-NHS forms a protective barrier that effectively prevents adhesion to surrounding normal tissues. Furthermore, the integration of PPy-GelMA significantly extends the degradation duration of the hydrogel, from 24 to 48 h. In a mouse model of intestinal perforation, the AA-A30 hydrogel demonstrated remarkable efficacy in inhibiting inflammation and preventing tissue adhesion by modulating the expression of both inflammatory and tissue adhesion-related factors, such as IL-1β, TNF-α, and the ratio of tPA to PAI-1. These findings underscore the considerable potential of AA-A30 for the therapeutic management of intestinal perforations.
肠穿孔是一种严重的医疗急症，传统手术常引发粘连等并发症。创新水凝胶凭借其抗粘连、抗菌和止血特性改善术后护理与康复。我们研发了一种先进的抗粘连水凝胶AA - A30，它由聚吡咯改性明胶（PPy - GelMA）、羧甲基壳聚糖（CMCS）和N - 羟基琥珀酰亚胺功能化聚乙二醇（PEG - NHS）组成。这种水凝胶是专门为肠穿孔量身定制的。水解后，PEG - NHS形成一层保护屏障，有效防止与周围正常组织粘连。此外，PPy - GelMA的加入显著延长了水凝胶的降解时间，从24小时延长至48小时。在小鼠肠穿孔模型中，AA - A30水凝胶通过调节炎症和组织粘连相关因子（如IL - 1β、TNF - α以及tPA与PAI - 1的比值）的表达，在抑制炎症和预防组织粘连方面显示出显著疗效。这些发现凸显了AA - A30在肠穿孔治疗管理方面的巨大潜力。
