College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China; Research Institute of Pharmaceutical Particle Technology, Zhejiang University of Technology, Hangzhou 310014, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127151. doi: 10.1016/j.ijbiomac.2023.127151. Epub 2023 Sep 29.
Microenvironment regeneration in wound tissue is crucial for wound healing. However, achieving desirable wound microenvironment regeneration involves multiple stages, including hemostasis, inflammation, proliferation, and remodeling. Traditional wound dressings face challenges in fully manipulating all these stages to achieve quick and complete wound healing. Herein, we present a VEGF-loaded, versatile wound dressing hydrogel based on gelatin methacryloyl (GelMA) and carboxymethyl chitosan (CMCS), which could be easily fabricated using UV irradiation. The newly designed GelMA-CMCS@VEGF hydrogel not only exhibited strong tissue adhesion capacity due to the interactions between CMCS active groups and biological tissues, but also possessed desirable extensible properties for frequently moving skins and joints. Furthermore, the hydrogel demonstrates exceptional abilities in blood cell coagulation, hemostasis and cell recruitment, leading to the promotion of endothelial cells proliferation, adhesion, migration and angiogenesis. Additionally, in vivo studies demonstrated that the hydrogel drastically shortened hemostatic time, and achieved satisfactory therapeutic efficacy by suppressing inflammation, modulating M1/M2 polarization of macrophages, significantly promoting collagen deposition, stimulating angiogenesis, epithelialization and tissue remodeling. This work contributes to the design of versatile hydrogel dressings for rapid and complete wound healing therapy.
创伤组织中的微环境再生对于创伤愈合至关重要。然而，实现理想的创伤微环境再生涉及多个阶段，包括止血、炎症、增殖和重塑。传统的创伤敷料在全面调控所有这些阶段以实现快速和完全的创伤愈合方面面临挑战。在此，我们提出了一种基于明胶甲基丙烯酰（GelMA）和羧甲基壳聚糖（CMCS）的负载 VEGF 的多功能创伤敷料水凝胶，它可以通过紫外光照射轻松制备。新设计的 GelMA-CMCS@VEGF 水凝胶不仅由于 CMCS 活性基团与生物组织之间的相互作用而表现出强大的组织黏附能力，而且还具有适合经常活动的皮肤和关节的可拉伸特性。此外，该水凝胶在血细胞凝结、止血和细胞募集方面表现出优异的能力，从而促进内皮细胞的增殖、黏附、迁移和血管生成。此外，体内研究表明，该水凝胶极大地缩短了止血时间，并通过抑制炎症、调节巨噬细胞的 M1/M2 极化、显著促进胶原蛋白沉积、刺激血管生成、上皮化和组织重塑，实现了令人满意的治疗效果。这项工作有助于设计用于快速和完全创伤愈合治疗的多功能水凝胶敷料。
