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传统药用植物乌药的染色体水平基因组组装

Chromosome-level genome assembly of the traditional medicinal plant Lindera aggregata.

作者信息

Shi Yujie, Chen Zhen, Ge Junxia, Jiang Jingyong, Li Qianfan, Lin Yiluo, Yu Weifu, Zeng Wei

机构信息

Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, College of Life Sciences, Taizhou University, Taizhou, 318000, China.

Zhejiang Hongshiliang Group Tiantai Mountain Wu-Yao Co., Ltd., Taizhou, 318000, China.

出版信息

Sci Data. 2025 Apr 3;12(1):565. doi: 10.1038/s41597-025-04891-3.

Abstract

Lindera aggregata is a renowned medicinal plant in China, particularly the variety from Tiantai, Zhejiang Province, which is esteemed for its superior medicinal properties. Beyond its medicinal value, it holds significant economic potential and phylogenetic significance. Utilizing a range of sequencing techniques, we have successfully assembled and annotated a high-quality chromosome-level genome of L. aggregata. The assembled genome spans approximately 1.59 Gb, with a scaffold N50 length of 132.62 Mb. Approximately 93.07% of the assembled sequences have been anchored to 12 pseudo-chromosomes, and 70.02% of the genome consists of repetitive sequences. According to the annotations, a total of 33,283 genes are identified, of which 96.95% can predict function. This high-quality chromosome-level assembly and annotation will greatly assist in the development and utilization of L. aggregata's valuable resources, and also provide a crucial molecular foundation for investigating the evolutionary relationships within the Lauraceae family and the mechanisms behind the synthesis of active ingredients in L. aggregata.

摘要

乌药是中国著名的药用植物,尤其是来自浙江天台的品种,因其卓越的药用特性而备受推崇。除了药用价值外,它还具有巨大的经济潜力和系统发育意义。利用一系列测序技术,我们成功地组装并注释了高质量的乌药染色体水平基因组。组装后的基因组大小约为1.59Gb,支架N50长度为132.62Mb。约93.07%的组装序列已被锚定到12条假染色体上,基因组的70.02%由重复序列组成。根据注释,共鉴定出33283个基因,其中96.95%的基因可以预测功能。这种高质量的染色体水平组装和注释将极大地有助于乌药宝贵资源的开发和利用,也为研究樟科内部的进化关系以及乌药活性成分合成机制提供关键的分子基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ca/11969015/061eafe0b619/41597_2025_4891_Fig1_HTML.jpg

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