[1,5] - 迁移重排反应可用于制备一种高度稳定的用于蛋白质自旋标记的螺环氮氧化物。
Sigmatropic rearrangement enables access to a highly stable spirocyclic nitroxide for protein spin labelling.
作者信息
Sowiński Mateusz P, Mocanu Elena M, Ruskin-Dodd Hannah, McKay Aidan P, Cordes David B, Lovett Janet E, Haugland-Grange Marius
机构信息
Department of Chemistry, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
SUPA School of Physics and Astronomy and BSRC, University of St Andrews, North Haugh, St Andrews KY16 9SS, UK.
出版信息
Chem Commun (Camb). 2025 May 1;61(37):6755-6758. doi: 10.1039/d5cc00472a.
Spin labelling enables the study of biomolecules using electron paramagnetic resonance (EPR) spectroscopy. Here, we describe the synthesis of a cysteine-reactive spin label based on a spirocyclic pyrrolidinyl nitroxide containing an iodoacetamide moiety. The spin label was shown to be highly persistent under reducing conditions while maintaining excellent EPR relaxation parameters up to a temperature of 180 K. After successful double spin labelling of a calmodulin variant, interspin distances were measured by the EPR experiment double electron-electron resonance (DEER) at 120 K.
自旋标记能够利用电子顺磁共振（EPR）光谱对生物分子进行研究。在此，我们描述了一种基于含有碘乙酰胺部分的螺环吡咯烷基氮氧化物的半胱氨酸反应性自旋标记的合成方法。该自旋标记在还原条件下表现出高度的稳定性，同时在高达180 K的温度下保持优异的EPR弛豫参数。在成功对钙调蛋白变体进行双自旋标记后，于120 K通过EPR实验双电子-电子共振（DEER）测量了自旋间距离。
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