Littlefield C L, Dunne J F, Bode H R
Dev Biol. 1985 Aug;110(2):308-20. doi: 10.1016/0012-1606(85)90090-9.
A morphological description of cells participating in sperm formation in Hydra oligactis males using a maceration procedure is presented. These descriptions are corroborated by the use of a monoclonal antibody, AC2, that binds to both a subpopulation of interstitial cells that appears to participate exclusively in gamete formation, and to all the gamete-differentiation products, including sperm intermediate cells, spermatids, and sperm. Use of the antibody as an interstitial cell marker has allowed an analysis of the behavior of the gamete-precursor (AC2+) subpopulation of interstitial cells during the asexual state and the early stages of gamete formation, when no differentiating sperm intermediates are present. The results indicate there is a gamete-producing subpopulation of interstitial cells which is present in low numbers in asexual males and undergoes extensive growth following the onset of spermatogenesis to give rise to sperm intermediate cells, and, eventually, the sperm. No input from the AC2- interstitial cells is required to account for this growth or subsequent sperm production. We speculate that the AC2+ interstitial cells may represent a unique subpopulation which is developmentally restricted to sperm production.
本文介绍了使用浸渍法对寡柄水螅雄性个体中参与精子形成的细胞进行的形态学描述。这些描述通过使用单克隆抗体AC2得到了证实，该抗体与似乎专门参与配子形成的间质细胞亚群以及所有配子分化产物（包括精子中间细胞、精子细胞和精子）结合。将该抗体用作间质细胞标记物，使得能够分析在无性状态和配子形成早期（此时不存在分化的精子中间细胞）期间间质细胞的配子前体（AC2+）亚群的行为。结果表明，存在一个产生配子的间质细胞亚群，其在无性雄性个体中数量较少，在精子发生开始后经历大量增殖，产生精子中间细胞，并最终产生精子。这种增殖或随后的精子产生不需要AC2-间质细胞的参与。我们推测，AC2+间质细胞可能代表一个独特的亚群，其发育仅限于精子产生。
