Eterman K P, Hekkens W T, Pena A S, Lems-van Kan P H, Feltkamp T E
J Immunol Methods. 1977;14(1):85-92. doi: 10.1016/s0022-1759(97)90025-9.
A simple method of detecting gluten antibodies in serum is described. Cryostat sections of wheat grains proved to be an excellent substrate in the immunofluorescence technique. Rabbit antisera to gliadin and alpha-gliadin, and high percentage of sera from patients with gluten-induced enteropathy had antibodies that reacted with an internal structure of wheat grains. These antibodies could be absorbed with gliadin and with alpha-gliadin.
本文描述了一种检测血清中麸质抗体的简单方法。小麦籽粒的低温恒温器切片在免疫荧光技术中被证明是一种优良的底物。抗麦醇溶蛋白和α-麦醇溶蛋白的兔抗血清,以及麸质诱发肠病患者的高比例血清中含有能与小麦籽粒内部结构发生反应的抗体。这些抗体可被麦醇溶蛋白和α-麦醇溶蛋白吸收。