Ogundare Tunde E, Casey Theresa M, Aryal Uma K, Fasina Yewande O
Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
Poult Sci. 2025 Mar 30;104(6):105091. doi: 10.1016/j.psj.2025.105091.
Salmonella infection severely impacts poultry health, productivity, and food safety. Spray dried plasma (SDP) shows good efficacy as an alternative to in-feed antibiotics for broiler chickens. Our objective was to identify potential mechanisms of SDP benefits by analyzing effects on spleen proteome and in response to Salmonella Enteritidis challenge (SE). A 2 × 2 factor study of broilers (n = 240) were assigned to CON (control) or SDP (30g/kg) diet at 1 day of age, and gavaged with sterile tryptic soy or 7.46 × 10 CFU SE /mL, resulting in 4 groups CON (n = 60), SDP (n = 60), CON-SE (n = 60) and SDP-SE (n = 60). On d 2 and d 5 post-challenge, chicks (n = 4/treatment) were euthanized, and spleen collected to isolate proteins, and measure using label-free liquid chromatography tandem mass spectrometry. Data were mapped to Gallus gallus Uniprot database, and differentially abundant proteins (DAP; P < 0.05) identified using ANOVA. Across all samples, 4,300 proteins were identified, and SDP treatment resulted 116 DAP. Proteins (56) that decreased were associated with positive regulation of I-kappaß kinase/NF- kappaß signaling and positive regulation of apoptosis process. Proteins that increased (60) were associated with integral components of endoplasmic reticulum membrane and necroptosis pathway. Analysis of the interaction between SDP and SE challenge found 119 DAP. The proteins that increased (87) were associated with RNA binding, ATP binding, mRNA splicing, and protein phosphorylation. Proteomic signatures of spleen reveals that the actions of SDP in broiler chicks are anti-inflammatory and anti-apoptotic and predicted to inhibit pro-inflammatory response through the inhibition of NF-ĸB. However, in the presence of Salmonella infection, it promotes regulated inflammatory response.
沙门氏菌感染严重影响家禽健康、生产力和食品安全。喷雾干燥血浆（SDP）作为肉鸡饲料中抗生素的替代品显示出良好的效果。我们的目标是通过分析对脾脏蛋白质组的影响以及对肠炎沙门氏菌攻击（SE）的反应来确定SDP益处的潜在机制。一项2×2因子研究中，240只肉鸡在1日龄时被分配到CON（对照）或SDP（30g/kg）日粮组，并分别灌喂无菌胰蛋白酶大豆肉汤或7.46×10 CFU SE/mL，从而形成4个组：CON（n = 60）、SDP（n = 60）、CON-SE（n = 60）和SDP-SE（n = 60）。在攻毒后第2天和第5天，每组处死4只雏鸡，采集脾脏分离蛋白质，并用无标记液相色谱串联质谱法进行测定。数据映射到家鸡Uniprot数据库，并使用方差分析确定差异丰度蛋白（DAP；P < 0.05）。在所有样本中，共鉴定出4300种蛋白质，SDP处理产生了116种DAP。减少的蛋白质（56种）与I-κB激酶/NF-κB信号的正调控和凋亡过程的正调控有关。增加的蛋白质（60种）与内质网膜的整合成分和坏死性凋亡途径有关。对SDP和SE攻击之间相互作用的分析发现了119种DAP。增加的蛋白质（87种）与RNA结合、ATP结合、mRNA剪接和蛋白质磷酸化有关。脾脏的蛋白质组学特征表明，SDP在肉鸡中的作用具有抗炎和抗凋亡作用，并预计通过抑制NF-κB来抑制促炎反应。然而，在沙门氏菌感染的情况下，它会促进调节性炎症反应。
