Adetunji Adedeji, Casey Theresa, Franco Jackeline, Shah Devendra, Fasina Yewande
Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
Molecules. 2022 Oct 26;27(21):7277. doi: 10.3390/molecules27217277.
Salmonella enteritidis is a foodborne pathogen that causes high morbidity in poultry. Proteomic analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to study the effects of Salmonella infection on spleen proteome in broiler chickens. Day-old broilers were assigned to control (CON; n = 60) or Salmonella challenge (CON−SE; n = 60), and gavaged with Tryptic soy agar broth or SE. A subset of chicks was euthanized on D3 and D7 (n = 4/group/day) and the spleen was removed, and rapidly frozen, subsequently proteome was measured using label-free LC-MS/MS. Protein spectra were mapped to Gallus gallus Uniprot database. Differentially abundant proteins (DAP; FDR < 0.05) between days and treatments were identified using ANOVA. Cecal content of Salmonella in CON−SE was 3.37 log10 CFU/g and CON were negative. Across the 16 samples, 2625 proteins were identified. Proteins that decreased in abundance between days mediated cell cycle progression, while those that increased in abundance function in cytoskeleton and mRNA processing. SE infection caused an increase in proteins that mediated redox homeostasis, lysosomal activities, and energy production, while proteins decreased in abundance-mediated developmental progression. Proteomic signatures of spleen suggest SE infection was metabolically costly, and energy was diverted from normal developmental processes to potentiate disease resistance mechanisms.
肠炎沙门氏菌是一种食源性病原体，可导致家禽的高发病率。采用液相色谱串联质谱法（LC-MS/MS）进行蛋白质组学分析，以研究沙门氏菌感染对肉鸡脾脏蛋白质组的影响。将一日龄肉鸡分为对照组（CON；n = 60）或沙门氏菌攻击组（CON−SE；n = 60），分别用胰蛋白胨大豆琼脂肉汤或肠炎沙门氏菌进行灌胃。在第3天和第7天对一部分雏鸡实施安乐死（每组每天n = 4），取出脾脏并迅速冷冻，随后使用无标记LC-MS/MS测量蛋白质组。将蛋白质谱映射到家鸡的Uniprot数据库。使用方差分析确定不同天数和处理之间差异丰富的蛋白质（DAP；FDR < 0.05）。CON−SE组盲肠内容物中的沙门氏菌为3.37 log10 CFU/g，CON组为阴性。在16个样本中，共鉴定出2625种蛋白质。丰度在不同天数之间降低的蛋白质介导细胞周期进程，而丰度增加的蛋白质在细胞骨架和mRNA加工中发挥作用。肠炎沙门氏菌感染导致介导氧化还原稳态、溶酶体活性和能量产生的蛋白质增加，而丰度降低的蛋白质介导发育进程。脾脏的蛋白质组特征表明，肠炎沙门氏菌感染在代谢上成本高昂，能量从正常发育过程中转移，以增强抗病机制。
