Bayer E A, Setter E, Lamed R
J Bacteriol. 1985 Aug;163(2):552-9. doi: 10.1128/jb.163.2.552-559.1985.
The properties of the cellulosome (the cellulose-binding, multicellulase-containing protein complex) in Clostridium thermocellum were examined by comparing the cellulase systems derived from the wild type and an adherence-defective mutant. The growth conditions--specifically, growth either on cellulose (Avicel) or on cellobiose as insoluble or soluble carbon sources, respectively--were found to be critical to the distribution of the cellulosome in the mutant system: the cellobiose-grown mutant (in contrast to the wild type) lacked the cellulosome on its surface and produced only minor quantities of the extracellular cellulosome accompanied by other relatively low-molecular-weight cellulases. The polypeptide composition of the respective purified cellulosome was dependent on the nature of the carbon source and was similar for both wild-type and mutant cells. Ultrastructural analysis revealed the presence of novel polycellulosomal protuberances on the cell surface of the cellobiose-grown wild type which were absent in the mutant.
通过比较来自野生型和黏附缺陷型突变体的纤维素酶系统，研究了嗜热栖热梭菌中纤维小体（纤维素结合的、含多种纤维素酶的蛋白质复合物）的特性。结果发现，生长条件——具体而言，分别以纤维素（微晶纤维素）或纤维二糖作为不溶性或可溶性碳源生长——对于突变体系统中纤维小体的分布至关重要：在纤维二糖上生长的突变体（与野生型相比）表面缺乏纤维小体，仅产生少量的细胞外纤维小体以及其他相对低分子量的纤维素酶。各自纯化的纤维小体的多肽组成取决于碳源的性质，野生型和突变体细胞的情况相似。超微结构分析显示，在以纤维二糖生长的野生型细胞表面存在新型的多纤维小体突起，而突变体中不存在。
