Imaging Intravoxel Vessel Size Distribution in the Brain Using Susceptibility Contrast Enhanced MRI.

Vascular remodelling is inherent to the pathogenesis of many diseases including cancer, neurodegeneration, fibrosis, hypertension, and diabetes. In this paper, a new susceptibility-contrast based MRI approach is established to non-invasively image intravoxel vessel size distribution (VSD), enabling a more comprehensive and quantitative assessment of vascular remodelling. The approach utilizes high-resolution light-sheet fluorescence microscopy images of rodent brain vasculature, simulating gradient echo sampling of free induction decay and spin echo (GESFIDE) MRI signals for the three-dimensional vascular networks, and training a deep learning model to predict cerebral blood volume (CBV) and VSD from GESFIDE signals. The results from experiments demonstrated strong correlation (r = 0.96) between the true and predicted CBV. High similarity between true and predicted VSDs was observed (mean Bhattacharya Coefficient = 0.92). With further validation, intravoxel VSD imaging could become a transformative preclinical and clinical tool for interrogating disease and treatment induced vascular remodelling.