Martin E T, Cote J A, Perry L K, Martin W J
J Clin Microbiol. 1985 Jul;22(1):19-22. doi: 10.1128/jcm.22.1.19-22.1985.
A bioluminescence method for screening urine cultures to provide rapid reporting of negative specimens and to select appropriate urine cultures for direct application of automated identification methods was evaluated. A total of 2,000 specimens were processed in the Lumac Biocounter (3M, St. Paul, Minn.), and the results were compared with quantitative culture techniques by using a 0.001-ml inoculating loop. A total of 841 specimens were positive by the bioluminescence method; 291 specimens were culture positive (greater than or equal to 50,000 CFU of one or two organisms per ml). Positive cultures represented more than 20 different organisms. Approximately two-thirds of the false-positive results represented mixed flora or pure cultures of less than 5 X 10(4) organisms per ml. The predictive value of a negative result was 98.4%, reflecting a false-negative rate of only 0.7%. No advantages in cost or technician time were noted, but the Lumac method appears to be a useful technique in decreasing reporting time, especially for negative urine cultures.
对一种用于筛查尿培养物的生物发光方法进行了评估，该方法可快速报告阴性标本，并选择合适的尿培养物直接应用自动鉴定方法。总共2000份标本在Lumac生物计数器（3M公司，明尼苏达州圣保罗）中进行处理，结果通过使用0.001 ml接种环与定量培养技术进行比较。生物发光法检测出841份标本呈阳性；291份标本培养阳性（每毫升一种或两种微生物≥50,000 CFU）。阳性培养物代表20多种不同的微生物。约三分之二的假阳性结果代表混合菌群或每毫升少于5×10⁴个微生物的纯培养物。阴性结果的预测值为98.4%，假阴性率仅为0.7%。未发现成本或技术人员时间方面的优势，但Lumac方法似乎是一种有用的技术，可减少报告时间，尤其是对于阴性尿培养物。
