Gilligan P H, Gage P A, Bradshaw L M, Schidlow D V, DeCicco B T
J Clin Microbiol. 1985 Jul;22(1):5-8. doi: 10.1128/jcm.22.1.5-8.1985.
A new medium for the isolation of Pseudomonas cepacia from respiratory tract secretions of patients with cystic fibrosis (CF) is described. This medium consists of inorganic salts, 0.5% pyruvate, and 0.1% proteose peptone as nutritive components and 0.0001% crystal violet, 0.15% bile salts, 100 micrograms of ticarcillin per ml, and 300 U of polymyxin B per ml as selective agents. The medium, designated PC medium, supported superior growth of 38 of 50 stock isolates of P. cepacia after 48 h of incubation when compared with MacConkey agar (0 of 50). The medium completely inhibited the growth of 112 of 124 stock isolates of organisms commonly found in respiratory secretions of CF patients. Cultures were made on PC medium with respiratory secretions of 169 CF patients. P. cepacia was recovered from 35 patients with isolates on PC medium but from only 21 patients with isolates on MacConkey agar. Of 221 other potentially pathogenic isolates found in these specimens, only six (two Pseudomonas aeruginosa isolates, two molds, one yeast, and one Serratia marcescens isolate) grew on PC medium. PC medium should facilitate the recovery of P. cepacia from CF patients.
本文描述了一种用于从囊性纤维化（CF）患者呼吸道分泌物中分离洋葱伯克霍尔德菌的新培养基。该培养基由无机盐、0.5%的丙酮酸和0.1%的蛋白胨作为营养成分，以及0.0001%的结晶紫、0.15%的胆盐、每毫升100微克的替卡西林和每毫升300单位的多粘菌素B作为选择剂组成。该培养基命名为PC培养基，与麦康凯琼脂（50株均未生长）相比，在孵育48小时后，50株洋葱伯克霍尔德菌标准菌株中有38株在PC培养基上生长良好。该培养基完全抑制了124株CF患者呼吸道分泌物中常见微生物标准菌株中112株的生长。用169例CF患者的呼吸道分泌物在PC培养基上进行培养。在PC培养基上从35例患者中分离出洋葱伯克霍尔德菌，而在麦康凯琼脂上仅从21例患者中分离出该菌。在这些标本中发现的221株其他潜在致病菌株中，只有6株（2株铜绿假单胞菌、2株霉菌、1株酵母菌和1株粘质沙雷氏菌）在PC培养基上生长。PC培养基应有助于从CF患者中分离出洋葱伯克霍尔德菌。
