Stover G B, Drake D R, Montie T C
Infect Immun. 1983 Sep;41(3):1099-104. doi: 10.1128/iai.41.3.1099-1104.1983.
The virulence of Pseudomonas aeruginosa and other pseudomonads was examined in a burned mouse model. P. aeruginosa M-2 was highly virulent causing 100% mortality by 38 h with an injection of 10(2) CFU by either a subcutaneous or intraperitoneal route. Subcutaneous injection of 10(2) CFU revealed rapid multiplication of the bacteria at the burn wound with 10(8) CFU/g detectable in the burned skin by 28 h postinjection, 10(5) CFU/g of liver, and 10(3) CFU/ml of blood. Non-P. aeruginosa clinical isolates were markedly less virulent; an injection of greater than or equal to 10(7) CFU caused less than or equal to 60% lethality. P. cepacia SMH colonized the burned skin of thermally injured mice, persisting at levels of 10(7) to 10(8) CFU/g of burned skin after an initial injection of 10(5) CFU. P. cepacia persisted in the burn wound for at least 3 weeks. No organ invasion was detectable throughout this period. Studies with an additional clinical isolate of P. cepacia yielded similar results. An injection of a 10(2) CFU dose revealed that the level of persistence is dose dependent. Results suggest that the tenacious persistence of P. cepacia in the burn wound may provide a model for the study of persistent colonization and infection in a compromised host.
在烧伤小鼠模型中检测了铜绿假单胞菌和其他假单胞菌的毒力。铜绿假单胞菌M-2具有高毒力，通过皮下或腹腔注射10²CFU，在38小时内可导致100%的死亡率。皮下注射10²CFU显示，细菌在烧伤创面迅速繁殖，注射后28小时在烧伤皮肤中可检测到10⁸CFU/g，肝脏中为10⁵CFU/g，血液中为10³CFU/ml。非铜绿假单胞菌临床分离株的毒力明显较低；注射大于或等于10⁷CFU导致的致死率小于或等于60%。洋葱伯克霍尔德菌SMH定殖于热损伤小鼠的烧伤皮肤，初次注射10⁵CFU后，在烧伤皮肤中的水平维持在10⁷至10⁸CFU/g。洋葱伯克霍尔德菌在烧伤创面持续存在至少3周。在此期间未检测到器官侵袭。对另一株洋葱伯克霍尔德菌临床分离株的研究得出了类似结果。注射10²CFU剂量显示，持续存在水平与剂量相关。结果表明，洋葱伯克霍尔德菌在烧伤创面的顽强持续存在可能为研究受损宿主中的持续定殖和感染提供一个模型。
