Vergari Alessandro, Polito Angelo, Musumeci Marco, Palazzesi Sergio, Marano Giuseppe
Istituto di Anestesia e Rianimazione, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Rome, Italy.
Lab Anim. 2003 Jul;37(3):204-6. doi: 10.1258/002367703766453047.
Orotracheal intubation in mice is a complicated technique because of the peculiar oropharyngeal anatomy and the difficulty in visualizing the laryngis aditus. Here we report a new and simple method for rapid endotracheal intubation by using a small bore, straight fibre-optic arthroscope. Under endoscope-assisted visualization of the laryngis aditus, a polyethylene cannula, inserted on a guide-wire in order to facilitate the introduction of the tip across the vocal cords, was advanced in the trachea. The success rate of intubation was 100%. We were also able to re-intubate the mice 4 and 8 weeks later without any major complications. We conclude that this method can be easily and safely used for studies where controlled pulmonary ventilation is necessary.
由于小鼠口咽解剖结构特殊且难以看清喉口，经口气管插管是一项复杂的技术。在此，我们报告一种使用小口径直型光纤关节镜进行快速气管插管的新的简易方法。在内镜辅助下可视化喉口，将一根聚乙烯套管套在导丝上，以便于套管尖端穿过声带进入气管。插管成功率为100%。我们还能够在4周和8周后再次为小鼠插管，且无任何严重并发症。我们得出结论，在需要控制肺通气的研究中，该方法可轻松、安全地使用。
