Yang Huaxin, Zheng Mengjia, Zhang Yuyue, Li Chaochang, Lai Joseph Ho Chi, Zhang Qizheng, Chan Kannie Wy, Wang Hao, Zhao Xin, Yang Zijiang, Xu Chenjie
Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China.
Center for Pluripotent Stem Cell Research and Engineering, Research Institute of Tsinghua, Guangzhou, Guangdong Province, China.
Biomater Transl. 2024 Mar 28;5(1):59-68. doi: 10.12336/biomatertransl.2024.01.006. eCollection 2024.
A composite scaffold composed of a porous scaffold and hydrogel filling can facilitate engraftment, survival, and retention in cell transplantation processes. This study presents a composite scaffold made of poly(ε-caprolactone) (PCL) and methacrylated hyaluronic acid (MeHA) hydrogel and describes the corresponding physical properties (surface area, porosity, and mechanical strength) and host response (angiogenesis and fibrosis) after subcutaneous transplantation. Specifically, we synthesise MeHA with different degrees of substitution and fabricate a PCL scaffold with different porosities. Subsequently, we construct a series of PCL/MeHA composite scaffolds by combining these hydrogels and scaffolds. In experiments with mice, the scaffold composed of 3% PCL and 10-100 kDa, degree of substitution 70% MeHA results in the least fibrosis and a higher degree of angiogenesis. This study highlights the potential of PCL/MeHA composite scaffolds for subcutaneous cell transplantation, given their desirable physical properties and host response.
由多孔支架和水凝胶填充物组成的复合支架能够促进细胞移植过程中的植入、存活和留存。本研究展示了一种由聚（ε-己内酯）（PCL）和甲基丙烯酸化透明质酸（MeHA）水凝胶制成的复合支架，并描述了皮下移植后的相应物理性质（表面积、孔隙率和机械强度）以及宿主反应（血管生成和纤维化）。具体而言，我们合成了不同取代度的MeHA，并制造了具有不同孔隙率的PCL支架。随后，我们通过将这些水凝胶和支架组合构建了一系列PCL/MeHA复合支架。在小鼠实验中，由3% PCL和10 - 100 kDa、取代度70%的MeHA组成的支架导致的纤维化最少且血管生成程度更高。鉴于其理想的物理性质和宿主反应，本研究突出了PCL/MeHA复合支架在皮下细胞移植中的潜力。
