Huang Xuan, Li Shibao, Tan Yuting, Xu Chunhui, Huang Yunyan, Yin Zhaozheng
Animal Science College, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang, 310058, China.
BMC Genomics. 2025 Jan 7;26(1):17. doi: 10.1186/s12864-024-11180-7.
The Taihe black-boned silky fowl, a distinguished indigenous breed of chicken, is renowned for its dual utility in both traditional medicinal and culinary applications. However, the breed faces significant challenges due to its suboptimal reproductive capabilities and a notably brief egg-laying period, which have impeded its broader development and cultivation. In this research endeavor, we employed an advanced, rapid DIA (Data independent acquisition) quantitative proteomics method on the Astral platform to meticulously analyze the ovarian proteome of these chickens. By analyzing the ovarian proteomic information of Taihe black-boned silky fowl during peak and decline egg-laying periods, we aim to identify potential reproductive candidate proteins and the molecular mechanisms underlying egg-laying decline. This could enable us to implement interventions to improve the reproductive efficiency of this valuable breed.
In this study, a total of 8,281 proteins were identified within the ovarian proteome of the Taihe black-boned silky fowl. Among these, 303 proteins exhibited significant differential expression, with 98 proteins significantly up-regulated and 205 proteins significantly down-regulated. The functional annotation of these proteins illuminated their crucial roles in the steroid hormone synthesis pathways, which are pivotal during the peak of egg production. Furthermore, during the later stages of laying, there was a noticeable upregulation of proteins associated with inflammatory senescence and oxidative stress. This change suggests an increase in reproductive stress within the ovary, highlighting the physiological shifts that affect productivity as the chickens age.
This study identified key candidate protein markers in the Taihe black-boned silky fowl during critical phases of their reproductive cycle, specifically peak and late egg-laying periods. These findings contribute valuable new scientific insights that can be utilized for the breeding optimization and effective management of this unique breed. By elucidating the protein dynamics during different laying phases, the research offers potential strategies aimed at enhancing reproductive performance and extending the reproductive lifespan of the Taihe black-boned silky fowl. This could lead to significant improvements in both the sustainability and profitability of farming this indigenous breed.
泰和乌骨鸡是一种著名的本土鸡品种，以其在传统药用和烹饪应用中的双重用途而闻名。然而，由于其繁殖能力欠佳且产蛋期明显较短，该品种面临着重大挑战，这阻碍了其更广泛的发展和养殖。在这项研究中，我们在Astral平台上采用了先进的快速数据非依赖采集（DIA）定量蛋白质组学方法，对这些鸡的卵巢蛋白质组进行了细致分析。通过分析泰和乌骨鸡产蛋高峰期和产蛋下降期的卵巢蛋白质组信息，我们旨在识别潜在的繁殖候选蛋白以及产蛋下降的分子机制。这可以使我们实施干预措施，以提高这个珍贵品种的繁殖效率。
在本研究中，泰和乌骨鸡的卵巢蛋白质组中共鉴定出8281种蛋白质。其中，303种蛋白质表现出显著差异表达，98种蛋白质显著上调，205种蛋白质显著下调。这些蛋白质的功能注释揭示了它们在类固醇激素合成途径中的关键作用，而类固醇激素合成途径在产蛋高峰期至关重要。此外，在产蛋后期，与炎症衰老和氧化应激相关的蛋白质出现明显上调。这种变化表明卵巢内繁殖应激增加，突出了随着鸡龄增长影响生产力的生理变化。
本研究在泰和乌骨鸡繁殖周期的关键阶段，即产蛋高峰期和后期，鉴定出了关键候选蛋白标志物。这些发现提供了有价值的新科学见解，可用于该独特品种的育种优化和有效管理。通过阐明不同产蛋阶段的蛋白质动态，该研究提供了旨在提高泰和乌骨鸡繁殖性能和延长其繁殖寿命的潜在策略。这可能会显著提高养殖这种本土品种的可持续性和盈利能力。
